caa a22 4500 469073470 CHVBK 20180323132928.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF01741052 doi (NATIONALLICENCE)springer-10.1007/BF01741052 Seroreactivity to HPV-16 proteins in women with early cervical neoplasia [Elektronische Daten] [Shannon Barber, John Werdel, Millie Symbula, John Williams, Barbara Burkett, Peyton Taylor, James Roche, Christopher Crum] Summary: Although serological reactivity to human papillomavirus type 16 (HPV-16) proteins has been demonstrated in patients with invasive cervical carcinoma, the degree of seroreactivity to these proteins in women with preinvasive disease and its relationship to the HPV type associated with the disease are unclear. We obtained sera from 27 women undergoing cone biopsy for cervical precursor lesions and 22 controls and analyzed seroreactivity by Western blot to fusion proteins containing portions of the HPV-16 E4, L1 and L2 open-reading frames (ORFs). Positives were analyzed by scanning densitometry and intensity values for each case plotted relative to controls. Cervical biopsy specimens from patients were analyzed for HPV-16 nucleic acids by DNA · DNA in situ hybridization. Mean intensity values for seroreactivity to the pATH-E4 protein approached significance (P = 0.058) and a significantly higher proportion of cases vs controls registered values over 4.0 for pATH-E4 (26% vs 4.5%;P = 0.04) and pATH-L2 (48% vs 18%;P = 0.03) proteins. A significantly higher mean intensity value for E4 was observed for cases containing HPV-16 DNA vs HPV-16 negative cases or controls. Thus, seroreactivity to HPV-16-derived proteins may be more common in women with preinvasive cervical disease, and for some protein targets (E4) may indicate a relatively type-specific response. Springer-Verlag, 1992 Fusion proteins nationallicence Papillomaviruses nationallicence Cervical dysplasia nationallicence Barber Shannon Departments of Pathology, Microbiology, Obstetrics and Gynecology, and Internal Medicine, University of Virginia Medical Center, Charlottesville, Va., USA aut Werdel John Departments of Pathology, Microbiology, Obstetrics and Gynecology, and Internal Medicine, University of Virginia Medical Center, Charlottesville, Va., USA aut Symbula Millie Departments of Pathology, Microbiology, Obstetrics and Gynecology, and Internal Medicine, University of Virginia Medical Center, Charlottesville, Va., USA aut Williams John Departments of Pathology, Microbiology, Obstetrics and Gynecology, and Internal Medicine, University of Virginia Medical Center, Charlottesville, Va., USA aut Burkett Barbara Departments of Pathology, Microbiology, Obstetrics and Gynecology, and Internal Medicine, University of Virginia Medical Center, Charlottesville, Va., USA aut Taylor Peyton Departments of Pathology, Microbiology, Obstetrics and Gynecology, and Internal Medicine, University of Virginia Medical Center, Charlottesville, Va., USA aut Roche James Departments of Pathology, Microbiology, Obstetrics and Gynecology, and Internal Medicine, University of Virginia Medical Center, Charlottesville, Va., USA aut Crum Christopher Departments of Pathology, Microbiology, Obstetrics and Gynecology, and Internal Medicine, University of Virginia Medical Center, Charlottesville, Va., USA aut Cancer Immunology, Immunotherapy Springer-Verlag 35/1(1992-01-01), 33-38 0340-7004 35:1<33 1992 35 262 https://doi.org/10.1007/BF01741052 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01741052 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barber Shannon Departments of Pathology, Microbiology, Obstetrics and Gynecology, and Internal Medicine, University of Virginia Medical Center, Charlottesville, Va., USA aut NATIONALLICENCE 700 1- Werdel John Departments of Pathology, Microbiology, Obstetrics and Gynecology, and Internal Medicine, University of Virginia Medical Center, Charlottesville, Va., USA aut NATIONALLICENCE 700 1- Symbula Millie Departments of Pathology, Microbiology, Obstetrics and Gynecology, and Internal Medicine, University of Virginia Medical Center, Charlottesville, Va., USA aut NATIONALLICENCE 700 1- Williams John Departments of Pathology, Microbiology, Obstetrics and Gynecology, and Internal Medicine, University of Virginia Medical Center, Charlottesville, Va., USA aut NATIONALLICENCE 700 1- Burkett Barbara Departments of Pathology, Microbiology, Obstetrics and Gynecology, and Internal Medicine, University of Virginia Medical Center, Charlottesville, Va., USA aut NATIONALLICENCE 700 1- Taylor Peyton Departments of Pathology, Microbiology, Obstetrics and Gynecology, and Internal Medicine, University of Virginia Medical Center, Charlottesville, Va., USA aut NATIONALLICENCE 700 1- Roche James Departments of Pathology, Microbiology, Obstetrics and Gynecology, and Internal Medicine, University of Virginia Medical Center, Charlottesville, Va., USA aut NATIONALLICENCE 700 1- Crum Christopher Departments of Pathology, Microbiology, Obstetrics and Gynecology, and Internal Medicine, University of Virginia Medical Center, Charlottesville, Va., USA aut NATIONALLICENCE 773 0- Cancer Immunology, Immunotherapy Springer-Verlag 35/1(1992-01-01), 33-38 0340-7004 35:1<33 1992 35 262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer