caa a22 4500 469073551 CHVBK 20180323132928.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF01789018 doi (NATIONALLICENCE)springer-10.1007/BF01789018 Effects of lipopolysaccharide on interleukin-2-induced cytotoxic activity of murine splenocyte cultures: role of prostaglandin E2 and interferons [Elektronische Daten] [Dominique Vaillier, Richard Daculsi, Norbert Gualde] Summary: Splenocytes cultured for 24 h in the presence of interleukin-2 (IL-2), lipopolysaccharide (LPS) or both together expressed a cytotoxic acitvity against the YAC-1 lymphoma cell line and to a lesser extent against P815 mastocytoma cells. The association of IL-2 and LPS had an additive effect on induction of cytotoxicity. The IL-2-induced cytotoxic activity lasted for the whole of the culture; however, the addition of LPS at the initiation of the culture increased the cytotoxic activity during its the early phase, the increment being followed by a fall of lytic activity after 72 h of culture. Assessment of interferon (IFN) in the culture supernatants showed (a) a production of IFNγ by IL-2-supplemented cultures, (b) a more potent IFN production by cultures treated with IL-2 plus LPS (including 20% IFNα/β), (c) and that indomethacin (1µm) potentiated the effect of either IL-2 or LPS used alone but did not significantly increase the cytotoxic activity of cultures treated with IL-2 plus LPS (the one that produced a high level of IFN). When cultures were treated by an anti-IFNγ antibody we observed no change in the cytotoxic activity; however, in the presence of anti-IFNα/β serum the cytotoxic activity of cultures treated with IL-2 plus LPS was inhibited after 24 h but stimulated after 72 h. When cultures treated with IL-2 plus LPS were supplemented with both indomethacin and anti-IFNα/β the cytotoxic activity assessed after 72 h of culture was maintained at the same level as that of IL-2-treated cultures, hence the fall after 72 h of the cytotoxicity of cultures initiated in the presence of LPS alone was affected by both the immune serum and the cyclooxygenase inhibitor. Altogether these data show that when splenocytes are cultured for more than 72 h in the presence of IL-2 and LPS their cytotoxic activity decreases, and it is likely that this diminution is linked to the endogenous prodution of prostaglandin E2 and IFNα/β. Springer-Verlag, 1992 Cytotoxicity nationallicence IL-2 nationallicence LPS nationallicence Interferons nationallicence Prostaglandins nationallicence Vaillier Dominique URA 1456 CNRS, Université de Bordeaux II, 146, rue Léo-Saignat, 33076, Bordeaux cedex, France aut Daculsi Richard URA 1456 CNRS, Université de Bordeaux II, 146, rue Léo-Saignat, 33076, Bordeaux cedex, France aut Gualde Norbert URA 1456 CNRS, Université de Bordeaux II, 146, rue Léo-Saignat, 33076, Bordeaux cedex, France aut Cancer Immunology, Immunotherapy Springer-Verlag 35/6(1992-11-01), 395-400 0340-7004 35:6<395 1992 35 262 https://doi.org/10.1007/BF01789018 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01789018 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vaillier Dominique URA 1456 CNRS, Université de Bordeaux II, 146, rue Léo-Saignat, 33076, Bordeaux cedex, France aut NATIONALLICENCE 700 1- Daculsi Richard URA 1456 CNRS, Université de Bordeaux II, 146, rue Léo-Saignat, 33076, Bordeaux cedex, France aut NATIONALLICENCE 700 1- Gualde Norbert URA 1456 CNRS, Université de Bordeaux II, 146, rue Léo-Saignat, 33076, Bordeaux cedex, France aut NATIONALLICENCE 773 0- Cancer Immunology, Immunotherapy Springer-Verlag 35/6(1992-11-01), 395-400 0340-7004 35:6<395 1992 35 262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer