caa a22 4500 46907356X CHVBK 20180323132928.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF01789014 doi (NATIONALLICENCE)springer-10.1007/BF01789014 Quantitative autoradiographic evaluation of the influence of protein dose on monoclonal antibody distribution in human ovarian adenocarcinoma xenografts [Elektronische Daten] [Farley Yang, Raya Brown, Ken Koral, Anaira Clavo, Gayle Jackson, Richard Wahl] Summary: We studied the effect of monoclonal antibody protein dose on the uniformity of radioiodinated antibody distribution within tumor masses using quantitative autoradiography. Groups (n = 11-13/group) of athymic nude mice with subcutaneous HTB77 human ovarian carcinoma xenografts were injected intraperitoneally with an125I-labeled anticarcinoma-associated antigen murine monoclonal antibody, 5G6.4, using a high or a low protein dose (500 µg or 5 µg). At 6 days post-injection the macroscopic and microscopic intratumoral biodistribution of radiolabeled antibody was determined. The degree of heterogeneity of the labeled antibody distribution within each tumor was quantified and expressed as thecoefficient of variation (CV) of the activity levels in serial histological sections. Tumors from mice given the 500-µg protein doses had substantially lower CV values, 0.327±0.027, than did tumors from animals given 5-µg protein doses, 0.458±0.041, (P = 0.0078), indicating that the higher protein dose resulted in more homogeneous distribution of radioactivity in tumors than did the lower dose. While the percentage of the injected dose reaching the tumor was comparable between groups, injecting the higher dose of protein resulted in significantly lower tumor to non-tumor uptake ratios than those obtained for the lower protein dose. These data indicate, in this system, that to achieve more uniform intratumoral antibody (and radiation for radioimmunotherapy) delivery, a relatively high protein dose must be administered. However, to obtain this increased uniformity, a substantial drop in tumor/background uptake ratios was seen. Quantitative autoradiographic evaluation of human tumor xenografts is a useful method to assess the intratumoral distribution of antibodies. Springer-Verlag, 1992 Monoclonal antibody nationallicence Ovarian carcinoma nationallicence Radiolabeled compounds nationallicence Quantitative autoradiography nationallicence Yang Farley Department of Internal Medicine, Division of Nuclear Medicine, University of Michigan Medical Center, 1500 E. Medical Center Drive, 48 109-0028, Ann Arbor, MI, USA aut Brown Raya Department of Internal Medicine, Division of Nuclear Medicine, University of Michigan Medical Center, 1500 E. Medical Center Drive, 48 109-0028, Ann Arbor, MI, USA aut Koral Ken Department of Internal Medicine, Division of Nuclear Medicine, University of Michigan Medical Center, 1500 E. Medical Center Drive, 48 109-0028, Ann Arbor, MI, USA aut Clavo Anaira Department of Internal Medicine, Division of Nuclear Medicine, University of Michigan Medical Center, 1500 E. Medical Center Drive, 48 109-0028, Ann Arbor, MI, USA aut Jackson Gayle Department of Internal Medicine, Division of Nuclear Medicine, University of Michigan Medical Center, 1500 E. Medical Center Drive, 48 109-0028, Ann Arbor, MI, USA aut Wahl Richard Department of Internal Medicine, Division of Nuclear Medicine, University of Michigan Medical Center, 1500 E. Medical Center Drive, 48 109-0028, Ann Arbor, MI, USA aut Cancer Immunology, Immunotherapy Springer-Verlag 35/6(1992-11-01), 365-372 0340-7004 35:6<365 1992 35 262 https://doi.org/10.1007/BF01789014 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01789014 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Farley Department of Internal Medicine, Division of Nuclear Medicine, University of Michigan Medical Center, 1500 E. Medical Center Drive, 48 109-0028, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Brown Raya Department of Internal Medicine, Division of Nuclear Medicine, University of Michigan Medical Center, 1500 E. Medical Center Drive, 48 109-0028, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Koral Ken Department of Internal Medicine, Division of Nuclear Medicine, University of Michigan Medical Center, 1500 E. Medical Center Drive, 48 109-0028, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Clavo Anaira Department of Internal Medicine, Division of Nuclear Medicine, University of Michigan Medical Center, 1500 E. Medical Center Drive, 48 109-0028, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Jackson Gayle Department of Internal Medicine, Division of Nuclear Medicine, University of Michigan Medical Center, 1500 E. Medical Center Drive, 48 109-0028, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Wahl Richard Department of Internal Medicine, Division of Nuclear Medicine, University of Michigan Medical Center, 1500 E. Medical Center Drive, 48 109-0028, Ann Arbor, MI, USA aut NATIONALLICENCE 773 0- Cancer Immunology, Immunotherapy Springer-Verlag 35/6(1992-11-01), 365-372 0340-7004 35:6<365 1992 35 262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer