caa a22 4500 469073578 CHVBK 20180323132928.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF01789022 doi (NATIONALLICENCE)springer-10.1007/BF01789022 T lymphocyte killing by a xanthine-oxidase-containing immunotoxin [Elektronische Daten] [M. Battelli, A. Abbondanza, P. Tazzari, A. Bolognesi, R. Lemoli, F. Stirpe] Summary: We report on the preparation of an immunotoxin consisting of xanthine oxidase, a free-radicalproducing enzyme, covalently linked to an anti-CD3 monoclonal antibody. The immunotoxin retained both enzymic and immunological properties and its toxicity to target cells (a) was greater than that of the free enzyme, (b) was proportional to the enzyme concentration, and (c) was reduced either in the absence of hypoxanthine or by an excess of free anti-CD3 monoclonal antibody. The cytotoxicity and selectivity of the hypoxanthine/conjugated xanthine oxidase system were potentiated by the addition of chelated iron and by washing away the unbound immunotoxin prior to the addition of substrate. The same system was not toxic to bone marrow progenitor cells. A possible use of this immunotoxin for the ex vivo purging of organs to be transplanted from T lymphocytes, to avoid the graft-versus-host reaction, is suggested. Springer-Verlag, 1992 Xanthine oxidase nationallicence Anti-CD3 monoclonal antibody nationallicence Immunotoxin nationallicence Battelli M. Department of Experimental Pathology, University of Bologna, Italy aut Abbondanza A. Department of Experimental Pathology, University of Bologna, Italy aut Tazzari P. Department of Experimental Pathology, University of Bologna, Italy aut Bolognesi A. Department of Experimental Pathology, University of Bologna, Italy aut Lemoli R. Institute of Haematology, University of Bologna, Italy aut Stirpe F. Department of Experimental Pathology, University of Bologna, Italy aut Cancer Immunology, Immunotherapy Springer-Verlag 35/6(1992-11-01), 421-425 0340-7004 35:6<421 1992 35 262 https://doi.org/10.1007/BF01789022 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01789022 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Battelli M. Department of Experimental Pathology, University of Bologna, Italy aut NATIONALLICENCE 700 1- Abbondanza A. Department of Experimental Pathology, University of Bologna, Italy aut NATIONALLICENCE 700 1- Tazzari P. Department of Experimental Pathology, University of Bologna, Italy aut NATIONALLICENCE 700 1- Bolognesi A. Department of Experimental Pathology, University of Bologna, Italy aut NATIONALLICENCE 700 1- Lemoli R. Institute of Haematology, University of Bologna, Italy aut NATIONALLICENCE 700 1- Stirpe F. Department of Experimental Pathology, University of Bologna, Italy aut NATIONALLICENCE 773 0- Cancer Immunology, Immunotherapy Springer-Verlag 35/6(1992-11-01), 421-425 0340-7004 35:6<421 1992 35 262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer