caa a22 4500 469073799 CHVBK 20180323132929.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF01756185 doi (NATIONALLICENCE)springer-10.1007/BF01756185 An extract of seeds from Aeginetia indica L., a parasitic plant, induces potent antigen-specific antitumor immunity in Meth A-bearing BALB/c mice [Elektronische Daten] [Jian-Guo Chai, Takashi Bando, Satoshi Kobashi, Mikio Oka, Hideyuki Nagasawa, Shoji Nakai, Ken-ichi Maeda, Kunisuke Himeno, Mitsunobu Sato, Shinya Ohkubo] Summary: The antitumor activity of an extract of seeds fromAeginetia indica L., a parasitic plant, was investigated. BALB/c mice, inoculated i.p. 1 × 105 syngeneic Meth A tumor cells, were administered 2.5 mg/kgA. indica extract i.p. every 2 days from day 0. The untreated mice died of an ascitic form of tumor growth within 21 days, whereas all the treated mice completely recovered from tumor challenge without any side-effects. The extract did not exert direct cytotoxic activity against Meth A in vitro. Mice that survived after the first challenge as a result ofA. indica treatment overcame the rechallenge with homologous Meth A without additional administration of the extract. On the other hand, those mice could not survive after rechallenge with Meth 1 tumor cells, which were also established in BALB/c mice but were different in antigenicity from Meth A, suggesting the development of antigen-specific concomitant immunity in theA. indica-cured mice. In the induction phase of antitumor resistance in this system, CD4+ T cells appeared to be the main contributors, since in vivo administration of anti-CD4 mAb completely abolished such resistance. In contrast, anti-CD8 mAb administration did not influence the effect ofA. indica. The importance of CD4+ T cells in antitumor immunity was again clarified by Winn assay; that is, spleen and lymph node cells depleted of CD4+ T cells in vitro prior to assay abolished antitumor activity on co-grafted Meth A tumor cells in vivo. Springer-Verlag, 1992 Aeginetia indica L nationallicence Meth A tumor nationallicence CD4+ T cells nationallicence Chai Jian-Guo Department of Parasitology and Immunology, School of Medicine, The University of Tokushima, Tokushima, Japan aut Bando Takashi Second Department of Oral and Maxillofacial Surgery, School of Dentistry, The University of Tokushima, Tokushima, Japan aut Kobashi Satoshi Department of Parasitology and Immunology, School of Medicine, The University of Tokushima, Tokushima, Japan aut Oka Mikio Department of Parasitology and Immunology, School of Medicine, The University of Tokushima, Tokushima, Japan aut Nagasawa Hideyuki Department of Parasitology and Immunology, School of Medicine, The University of Tokushima, Tokushima, Japan aut Nakai Shoji Department of Parasitology and Immunology, School of Medicine, The University of Tokushima, Tokushima, Japan aut Maeda Ken-ichi Department of Parasitology and Immunology, School of Medicine, The University of Tokushima, Tokushima, Japan aut Himeno Kunisuke Department of Parasitology and Immunology, School of Medicine, The University of Tokushima, Tokushima, Japan aut Sato Mitsunobu Second Department of Oral and Maxillofacial Surgery, School of Dentistry, The University of Tokushima, Tokushima, Japan aut Ohkubo Shinya Ohkubo Hospital, Tokushima, Japan aut Cancer Immunology, Immunotherapy Springer-Verlag 35/3(1992-05-01), 181-185 0340-7004 35:3<181 1992 35 262 https://doi.org/10.1007/BF01756185 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01756185 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chai Jian-Guo Department of Parasitology and Immunology, School of Medicine, The University of Tokushima, Tokushima, Japan aut NATIONALLICENCE 700 1- Bando Takashi Second Department of Oral and Maxillofacial Surgery, School of Dentistry, The University of Tokushima, Tokushima, Japan aut NATIONALLICENCE 700 1- Kobashi Satoshi Department of Parasitology and Immunology, School of Medicine, The University of Tokushima, Tokushima, Japan aut NATIONALLICENCE 700 1- Oka Mikio Department of Parasitology and Immunology, School of Medicine, The University of Tokushima, Tokushima, Japan aut NATIONALLICENCE 700 1- Nagasawa Hideyuki Department of Parasitology and Immunology, School of Medicine, The University of Tokushima, Tokushima, Japan aut NATIONALLICENCE 700 1- Nakai Shoji Department of Parasitology and Immunology, School of Medicine, The University of Tokushima, Tokushima, Japan aut NATIONALLICENCE 700 1- Maeda Ken-ichi Department of Parasitology and Immunology, School of Medicine, The University of Tokushima, Tokushima, Japan aut NATIONALLICENCE 700 1- Himeno Kunisuke Department of Parasitology and Immunology, School of Medicine, The University of Tokushima, Tokushima, Japan aut NATIONALLICENCE 700 1- Sato Mitsunobu Second Department of Oral and Maxillofacial Surgery, School of Dentistry, The University of Tokushima, Tokushima, Japan aut NATIONALLICENCE 700 1- Ohkubo Shinya Ohkubo Hospital, Tokushima, Japan aut NATIONALLICENCE 773 0- Cancer Immunology, Immunotherapy Springer-Verlag 35/3(1992-05-01), 181-185 0340-7004 35:3<181 1992 35 262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer