caa a22 4500 469073845 CHVBK 20180323132929.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF01789336 doi (NATIONALLICENCE)springer-10.1007/BF01789336 Immunoscintigraphic localization of renal tumours in an extracorporeal perfusion model with a monoclonal antibody against γ-glutamyltransferase [Elektronische Daten] [Peter Fischer, Richard Baum, Monika Tauber, Wieland Boeckmann, Stefan Weier, Jürgen Scherberich] Summary: Monoclonal antibody 138H11 against human γ-glutamyltransferase has been shown to react immunohistochemically with 98% of all tested clear-cell type and chromophilic renal cell carcinomas, but not with renal chromophobic carcinomas, Duct-Bellini carcinomas or oncocytomas. In normal kidney the target epitopes of mAb 138H11 are located in the luminal brush-border membrane of proximal tubule cells, whereas in renal carcinomas the epitopes are found surrounding the whole tumour cells. These results form the basis of the present immunoscintigraphic study designed to evaluate mAb 138H11 in an extracorporeal perfusion model. Immediately after nephrectomy, human tumour-bearing kidneys were perfused with99mTc-labelled mAb 138H11 in Euro-Collins solution. High specific uptake in 4/4 renal clear cell carcinomas could be demonstrated by planar immunoscintigraphy and single-photon-emission computed tomography, "regions of interest” investigation and immunohistochemistry. In contrast, a perfused oncocytoma showed up as an unlabelled lesion. The results indicate a possible use for mAb 138H11 in immunoscintigraphy or even therapy, provided high tumour uptake can be confirmed in patients. Springer-Verlag, 1992 Renal cell carcinoma nationallicence Immunoscintigraphy nationallicence Monoclonal antibody nationallicence γ-Glutamyltransferase nationallicence EC 2.3.2.2 nationallicence Fischer Peter Department of Nephrology, University Hospital, J. W. Goethe University, Theodor-Stern-Kai 7, W-6000, Frankfurt (Main) 70, Germany aut Baum Richard Department of Nuclear Medicine, University Hospital, J. W. Goethe University, Theodor-Stern-Kai 7, W-6000, Frankfurt (Main) 70, Germany aut Tauber Monika Department of Nephrology, University Hospital, J. W. Goethe University, Theodor-Stern-Kai 7, W-6000, Frankfurt (Main) 70, Germany aut Boeckmann Wieland Department of Urology, University Hospital, J. W. Goethe University, Theodor-Stern-Kai 7, W-6000, Frankfurt (Main) 70, Germany aut Weier Stefan Department of Pathology, University Hospital, J. W. Goethe University, Theodor-Stern-Kai 7, W-6000, Frankfurt (Main) 70, Germany aut Scherberich Jürgen Department of Nephrology, University Hospital, J. W. Goethe University, Theodor-Stern-Kai 7, W-6000, Frankfurt (Main) 70, Germany aut Cancer Immunology, Immunotherapy Springer-Verlag 35/4(1992-07-01), 283-288 0340-7004 35:4<283 1992 35 262 https://doi.org/10.1007/BF01789336 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01789336 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fischer Peter Department of Nephrology, University Hospital, J. W. Goethe University, Theodor-Stern-Kai 7, W-6000, Frankfurt (Main) 70, Germany aut NATIONALLICENCE 700 1- Baum Richard Department of Nuclear Medicine, University Hospital, J. W. Goethe University, Theodor-Stern-Kai 7, W-6000, Frankfurt (Main) 70, Germany aut NATIONALLICENCE 700 1- Tauber Monika Department of Nephrology, University Hospital, J. W. Goethe University, Theodor-Stern-Kai 7, W-6000, Frankfurt (Main) 70, Germany aut NATIONALLICENCE 700 1- Boeckmann Wieland Department of Urology, University Hospital, J. W. Goethe University, Theodor-Stern-Kai 7, W-6000, Frankfurt (Main) 70, Germany aut NATIONALLICENCE 700 1- Weier Stefan Department of Pathology, University Hospital, J. W. Goethe University, Theodor-Stern-Kai 7, W-6000, Frankfurt (Main) 70, Germany aut NATIONALLICENCE 700 1- Scherberich Jürgen Department of Nephrology, University Hospital, J. W. Goethe University, Theodor-Stern-Kai 7, W-6000, Frankfurt (Main) 70, Germany aut NATIONALLICENCE 773 0- Cancer Immunology, Immunotherapy Springer-Verlag 35/4(1992-07-01), 283-288 0340-7004 35:4<283 1992 35 262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer