caa a22 4500 469073993 CHVBK 20180323132930.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF01741144 doi (NATIONALLICENCE)springer-10.1007/BF01741144 Modulation of the antigenic phenotype of human breast carcinoma cells by modifiers of protein kinase C activity and recombinant human interferons [Elektronische Daten] [Jorge Leon, M. Gutierrez, Hongping Jiang, Alison Estabrook, Samuel Waxman, Paul Fisher] Summary: In the present study we have analyzed the effect of a synthetic protein kinase C (PKC) activator 3-(N-acetylamino)-5-(N-decyl-N-methylamino)-benzyl alcohol (ADMB) and the natural PKC-activating tumor-promoting agents 12-O-tetradecanoylphorbol 13-acetate (TPA) and mezerein on the antigenic phenotype of T47D human breast carcinoma cells. All three agents increased the surface expression of the tumor-associated antigen BCA 225 and various cellular antigens, including HLA class II antigens, intercellular adhesion molecule 1 (ICAM-1) and c-erbB-2. Expression of the same antigens was also upregulated to various extents in T47D cells by recombinant fibroblast (IFNβ) and immune (IFNγ) interferon. Shedding of BCA 225 from T47D cells was induced by TPA, mezerein, IFNβ and IFNγ, whereas ADMB did not display this activity. The ability of ADMB, TPA and mezerein to modulate the antigenic phenotype of T47D cells appears to involve a PKC-mediated pathway, since the PKC inhibitor, H-7, eliminates antigenic modulation. In contrast, the ability of IFNβ and IFNγ to enhance the synthesis, expression and shedding of BCA 225, as well as to enhance HLA class II antigens, c-erbB-2 and ICAM-1 expression, was either unchanged or modestly reduced by simultaneous exposure to H-7. Analysis of steady-state mRNA levels for HLA class I antigens, HLA class II-DRβ antigen, ICAM-1 and c-erbB-2 indicated that the ability of H-7 to inhibit expression of these antigens in TPA-, mezerein- and ADMB-treated cells was not a consequence of a reduction in the steady-state levels of mRNAs for these antigens. The results of the present investigation indicate that the biochemical pathways mediating enhanced antigenic expression in T47D cells induced by TPA, mezerein and the synthetic PKC activator ADMB are different from those induced by recombinant interferons. Furthermore, up-regulation of antigenic expression in T47D cells can occur by a PKC-dependent or a PKC-independent pathway. Springer-Verlag, 1992 Human breast carcinoma cells nationallicence Protein kinase C activators nationallicence Recombinant interferons nationallicence Antigenic phenotype nationallicence Tumor antigen shedding nationallicence Leon Jorge Department of Surgery, Columbia University, College of Physicians and Surgeons, 10032, New York, New York, USA aut Gutierrez M. Department of Pathology, Columbia University, College of Physicians and Surgeons, 630 West 168th Street, 10 032, New York, New York, USA aut Jiang Hongping Department of Pathology, Columbia University, College of Physicians and Surgeons, 630 West 168th Street, 10 032, New York, New York, USA aut Estabrook Alison Department of Surgery, Columbia University, College of Physicians and Surgeons, 10032, New York, New York, USA aut Waxman Samuel Department of Medicine, Mount Sinai School of Medicine, 10 029, New York, New York, USA aut Fisher Paul Department of Pathology, Columbia University, College of Physicians and Surgeons, 630 West 168th Street, 10 032, New York, New York, USA aut Cancer Immunology, Immunotherapy Springer-Verlag 35/5(1992-09-01), 315-324 0340-7004 35:5<315 1992 35 262 https://doi.org/10.1007/BF01741144 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01741144 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Leon Jorge Department of Surgery, Columbia University, College of Physicians and Surgeons, 10032, New York, New York, USA aut NATIONALLICENCE 700 1- Gutierrez M. Department of Pathology, Columbia University, College of Physicians and Surgeons, 630 West 168th Street, 10 032, New York, New York, USA aut NATIONALLICENCE 700 1- Jiang Hongping Department of Pathology, Columbia University, College of Physicians and Surgeons, 630 West 168th Street, 10 032, New York, New York, USA aut NATIONALLICENCE 700 1- Estabrook Alison Department of Surgery, Columbia University, College of Physicians and Surgeons, 10032, New York, New York, USA aut NATIONALLICENCE 700 1- Waxman Samuel Department of Medicine, Mount Sinai School of Medicine, 10 029, New York, New York, USA aut NATIONALLICENCE 700 1- Fisher Paul Department of Pathology, Columbia University, College of Physicians and Surgeons, 630 West 168th Street, 10 032, New York, New York, USA aut NATIONALLICENCE 773 0- Cancer Immunology, Immunotherapy Springer-Verlag 35/5(1992-09-01), 315-324 0340-7004 35:5<315 1992 35 262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer