caa a22 4500 469074043 CHVBK 20180323132930.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF01741856 doi (NATIONALLICENCE)springer-10.1007/BF01741856 Interleukin-6 and renal cell cancer: Production, regulation, and growth effects [Elektronische Daten] [Alec Koo, Cathy Armstrong, Bernard Bochner, Tomoyuki Shimabukuro, Cho-Lea Tso, Jean deKernion, Arie Belldegrun] Summary: Interleukin-6 (IL-6) is a recently characterized pleiotropic cytokine with antitumor activity. We investigated the production of IL-6 by renal cell cancer (RCC) and the growth effects of IL-6 on RCC. Using immunoperoxidase staining, cytoplasmic IL-6 was detected in four of four renal tumor lines and in tumor cells from freshly nephrectomized RCC. We found that IL-6 mRNA was expressed at basal culture conditions by seven of ten RCC tumor lines tested. Biologically active IL-6, as measured by the B9 assay, was produced by all ten RCC tumor lines. The addition of tumor necrosis factor α (TNFα) significantly augmented the expression of IL-6 mRNA in five RCC tumor lines (P <0.05). The combination of interferon γ IFNγ and TNFα further enhanced the augmented IL-6 mRNA accumulation seen with TNFα alone (P <0.05). TNFα also significantly stimulated the production of biologically active IL-6 (P <0.01). Furthermore, IFNγ and TNFα were found to enhance IL-6 bioactivity synergistically (P <0.05). The growth effects of IL-6 on RCC were also investigated in two experimental systems: IL-6 was found to stimulate proliferative responses in six of six RCC tumor lines as measured by thymidine-uptake assays; however, only one of six tumor lines displayed an increase in proliferative response of greater than 21% (113%). The growth effect of IL-6 was further tested in clonogenic assays. One of the tumor lines tested displayed an enhanced growth response of up to 200%. We conclude that IL-6 is produced by RCC; this production is enhanced by TNFα with synergistic effects seen with IFNγ at both mRNA and protein levels. In turn, IL-6 may have a modest stimulatory growth effect on certain RCC tumor lines. Springer-Verlag, 1992 Interleukin-6 nationallicence Renal cell cancer nationallicence Growth effect nationallicence Koo Alec Immunotherapy Laboratory, Division of Urology, Department of Surgery, and the Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, California, USA aut Armstrong Cathy Immunotherapy Laboratory, Division of Urology, Department of Surgery, and the Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, California, USA aut Bochner Bernard Immunotherapy Laboratory, Division of Urology, Department of Surgery, and the Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, California, USA aut Shimabukuro Tomoyuki Immunotherapy Laboratory, Division of Urology, Department of Surgery, and the Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, California, USA aut Tso Cho-Lea Immunotherapy Laboratory, Division of Urology, Department of Surgery, and the Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, California, USA aut deKernion Jean Immunotherapy Laboratory, Division of Urology, Department of Surgery, and the Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, California, USA aut Belldegrun Arie Immunotherapy Laboratory, Division of Urology, Department of Surgery, and the Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, California, USA aut Cancer Immunology, Immunotherapy Springer-Verlag 35/2(1992-03-01), 97-105 0340-7004 35:2<97 1992 35 262 https://doi.org/10.1007/BF01741856 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01741856 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koo Alec Immunotherapy Laboratory, Division of Urology, Department of Surgery, and the Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, California, USA aut NATIONALLICENCE 700 1- Armstrong Cathy Immunotherapy Laboratory, Division of Urology, Department of Surgery, and the Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, California, USA aut NATIONALLICENCE 700 1- Bochner Bernard Immunotherapy Laboratory, Division of Urology, Department of Surgery, and the Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, California, USA aut NATIONALLICENCE 700 1- Shimabukuro Tomoyuki Immunotherapy Laboratory, Division of Urology, Department of Surgery, and the Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, California, USA aut NATIONALLICENCE 700 1- Tso Cho-Lea Immunotherapy Laboratory, Division of Urology, Department of Surgery, and the Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, California, USA aut NATIONALLICENCE 700 1- deKernion Jean Immunotherapy Laboratory, Division of Urology, Department of Surgery, and the Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, California, USA aut NATIONALLICENCE 700 1- Belldegrun Arie Immunotherapy Laboratory, Division of Urology, Department of Surgery, and the Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, California, USA aut NATIONALLICENCE 773 0- Cancer Immunology, Immunotherapy Springer-Verlag 35/2(1992-03-01), 97-105 0340-7004 35:2<97 1992 35 262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer