caa a22 4500 469074086 CHVBK 20180323132930.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF01741854 doi (NATIONALLICENCE)springer-10.1007/BF01741854 Induction of interleukin-2 receptor by tumor necrosis factor α on cultured ovarian tumor-associated lymphocytes [Elektronische Daten] [Constantin Ioannides, Bryan Fisk, Barbara Tomasovic, Raj Pandita, Bharat Aggarwal, Ralph Freedman] Summary: We have recently reported that autologous tumor-specific cytotoxic T lymphocyte (CTL) lines and clones can be developed from lymphocytes infiltrating ovarian malignant ascites (TAL). In this study, we investigated the biological effects of tumor necrosis factor α (TNFα) in the induction, expansion, long-term proliferation and lytic function of CD8+ TAL. TNFα up-regulated the IL-2 receptor (IL-2R) α chain (Tac antigen) on the surface of CD3+ CD8+ CD4− TAL, enhanced the proliferation of autologous tumor-specific CTL, and potentiated their lytic function in long-term cultures. Furthermore, in the induction and expansion phase of CD8+ TAL, the presence of TNFα was associated with a selective increase in CD8+ IL-2R+ (Tac+) cells, and subsequent decrease in CD4+ IL-2R+ (Tac+) cells. These results suggest that the observed facilitation of the outgrowth of CD8+ cells in TAL cultures may be due, at least in part, to the up-regulation of IL-2R, and indicate the usefulness of TNFα in the analysis of signalling in autologous tumor-reactive CTL. Springer-Verlag, 1992 TNFα nationallicence CD8+ CTL nationallicence IL-2R nationallicence TIL/TAL nationallicence Ioannides Constantin Department of Gynecology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Box 67, 77 030, Houston, Texas, USA aut Fisk Bryan Department of Gynecology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Box 67, 77 030, Houston, Texas, USA aut Tomasovic Barbara Department of Gynecology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Box 67, 77 030, Houston, Texas, USA aut Pandita Raj Cytokine Research Laboratory, Department of Clinical Immunology and Biological Therapy, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, 77 030, Houston, Texas, USA aut Aggarwal Bharat Cytokine Research Laboratory, Department of Clinical Immunology and Biological Therapy, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, 77 030, Houston, Texas, USA aut Freedman Ralph Department of Gynecology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Box 67, 77 030, Houston, Texas, USA aut Cancer Immunology, Immunotherapy Springer-Verlag 35/2(1992-03-01), 83-91 0340-7004 35:2<83 1992 35 262 https://doi.org/10.1007/BF01741854 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01741854 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ioannides Constantin Department of Gynecology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Box 67, 77 030, Houston, Texas, USA aut NATIONALLICENCE 700 1- Fisk Bryan Department of Gynecology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Box 67, 77 030, Houston, Texas, USA aut NATIONALLICENCE 700 1- Tomasovic Barbara Department of Gynecology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Box 67, 77 030, Houston, Texas, USA aut NATIONALLICENCE 700 1- Pandita Raj Cytokine Research Laboratory, Department of Clinical Immunology and Biological Therapy, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, 77 030, Houston, Texas, USA aut NATIONALLICENCE 700 1- Aggarwal Bharat Cytokine Research Laboratory, Department of Clinical Immunology and Biological Therapy, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, 77 030, Houston, Texas, USA aut NATIONALLICENCE 700 1- Freedman Ralph Department of Gynecology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Box 67, 77 030, Houston, Texas, USA aut NATIONALLICENCE 773 0- Cancer Immunology, Immunotherapy Springer-Verlag 35/2(1992-03-01), 83-91 0340-7004 35:2<83 1992 35 262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer