caa a22 4500 469074140 CHVBK 20180323132930.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF01741752 doi (NATIONALLICENCE)springer-10.1007/BF01741752 Anti-(human LFA-1) monoclonal antibodies bind P815 murine tumour cells [Elektronische Daten] [Marie-José Palisson, Anne Altemeyer, Isabelle Moosbrugger, Stéphanie Warter, Georges Hauptmann, Pierre Bischoff] Summary: Using anti-CD11a and anti-CD18 monoclonal antibodies (mAbs) directed respectively against the α and the β chains of LFA-1, we obtained an important and specific staining of P815 murine tumour cells. Both ascitic and cultured cells displayed a positive staining. Other murine tumours of haematopoietic origin, as well as lymphocytes or lymphoblasts from DBA/2 mice, were not labelled by the same monoclonal antibodies. These results were surprising since, to our knowledge, no case of cross-reaction between species has been reported with LFA-1. Moreover, competition assays showed that epitopes recognized by the two anti-CD11a antibodies were different from those identified by H35.89.9, a mAb raised against the murine LFA-1 α chain. Using allogeneic cytotoxic T lymphocytes, we also showed that anti-(human LFA-1) mAbs were unable to block the lysis of P815 by these effector cells. Thus, the putative functional properties of these structures, as well as their importance from an antigeneic point of view, remain to be assessed. Springer-Verlag, 1992 Monoclonal antibodies nationallicence LFA-1 nationallicence Murine tumour nationallicence Palisson Marie-José Laboratoire de Recherches en Immunologie, Institut d'Hématologie et d'Immunologie, 1 Place de l'Hôpital, F-67 091, Strasbourg, France aut Altemeyer Anne Laboratoire de Recherches en Immunologie, Institut d'Hématologie et d'Immunologie, 1 Place de l'Hôpital, F-67 091, Strasbourg, France aut Moosbrugger Isabelle Laboratoire de Recherches en Immunologie, Institut d'Hématologie et d'Immunologie, 1 Place de l'Hôpital, F-67 091, Strasbourg, France aut Warter Stéphanie Laboratoire d'Embryologie, Faculté de Médecine, 4, rue Kirschleger, F-67 085, Strasbourg, France aut Hauptmann Georges Laboratoire de Recherches en Immunologie, Institut d'Hématologie et d'Immunologie, 1 Place de l'Hôpital, F-67 091, Strasbourg, France aut Bischoff Pierre Laboratoire de Recherches en Immunologie, Institut d'Hématologie et d'Immunologie, 1 Place de l'Hôpital, F-67 091, Strasbourg, France aut Cancer Immunology, Immunotherapy Springer-Verlag 34/6(1992-11-01), 407-413 0340-7004 34:6<407 1992 34 262 https://doi.org/10.1007/BF01741752 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01741752 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Palisson Marie-José Laboratoire de Recherches en Immunologie, Institut d'Hématologie et d'Immunologie, 1 Place de l'Hôpital, F-67 091, Strasbourg, France aut NATIONALLICENCE 700 1- Altemeyer Anne Laboratoire de Recherches en Immunologie, Institut d'Hématologie et d'Immunologie, 1 Place de l'Hôpital, F-67 091, Strasbourg, France aut NATIONALLICENCE 700 1- Moosbrugger Isabelle Laboratoire de Recherches en Immunologie, Institut d'Hématologie et d'Immunologie, 1 Place de l'Hôpital, F-67 091, Strasbourg, France aut NATIONALLICENCE 700 1- Warter Stéphanie Laboratoire d'Embryologie, Faculté de Médecine, 4, rue Kirschleger, F-67 085, Strasbourg, France aut NATIONALLICENCE 700 1- Hauptmann Georges Laboratoire de Recherches en Immunologie, Institut d'Hématologie et d'Immunologie, 1 Place de l'Hôpital, F-67 091, Strasbourg, France aut NATIONALLICENCE 700 1- Bischoff Pierre Laboratoire de Recherches en Immunologie, Institut d'Hématologie et d'Immunologie, 1 Place de l'Hôpital, F-67 091, Strasbourg, France aut NATIONALLICENCE 773 0- Cancer Immunology, Immunotherapy Springer-Verlag 34/6(1992-11-01), 407-413 0340-7004 34:6<407 1992 34 262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer