caa a22 4500 46907423X CHVBK 20180323132930.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF01741750 doi (NATIONALLICENCE)springer-10.1007/BF01741750 Role of interferon γ and tumour necrosis factor α in monocyte-mediated cytostasis and cytotoxicity against a human histiocytic lymphoma cell line [Elektronische Daten] [Arjan van de Loosdrecht, Gert Ossenkoppele, Robert Beelen, Marjolein Broekhoven, Mart Langenhuijsen] Summary: In view of cellular adoptive immunotherapy we have studied monocyte-mediated cytostasis and cytotoxicity against U 937 cells, a human histiocytic lymphoma cell line. Highly purified human monocytes and monocytederived macrophages were activated with interferon γ (IFN) or tumour necrosis factor α (TNF) to antileukemic immune effector cells. Antileukemic activity of human monocytes was dependent on monocyte differentiation into macrophages and on a dose- and time-dependent activation with IFN or TNF. Maximum cytostasis of 97.0±0.7% (mean ± SEM) (conventional [3H]dT uptake assay) and 81.9±5.3% cytotoxicity (modified MTT assay) of U 937 cells was obtained by monocytes activated with 100 U/ml IFN for at least 24 h at an effector-to-target-cell ratio of 10. U 937 cells premodified with IFN showed an increase in susceptibility to monocyte-mediated cytotoxicity. U 937 cells premodified with TNF were almost resistant to monocyte-mediated cytotoxicity while activated monocytes maintained their cytotoxic potential. These data show that IFN and TNF are potent activators of monocyte-mediated cytotoxicity. Furthermore, IFN and TNF might be involved in the regulation of the susceptibility of leukemic cells to lysis by interactions with monocytes or macrophages. Springer-Verlag, 1992 Monocytes nationallicence Leukemia nationallicence Cytotoxicity nationallicence IFNγ nationallicence TNFα nationallicence van de Loosdrecht Arjan Department of Haematology (BR238), University Hospital and Faculty of Medicine, Free University, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands aut Ossenkoppele Gert Department of Haematology (BR238), University Hospital and Faculty of Medicine, Free University, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands aut Beelen Robert Department of Cell Biology, University Hospital and Faculty of Medicine, Free University, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands aut Broekhoven Marjolein Department of Haematology (BR238), University Hospital and Faculty of Medicine, Free University, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands aut Langenhuijsen Mart Department of Haematology (BR238), University Hospital and Faculty of Medicine, Free University, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands aut Cancer Immunology, Immunotherapy Springer-Verlag 34/6(1992-11-01), 393-398 0340-7004 34:6<393 1992 34 262 https://doi.org/10.1007/BF01741750 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01741750 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van de Loosdrecht Arjan Department of Haematology (BR238), University Hospital and Faculty of Medicine, Free University, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Ossenkoppele Gert Department of Haematology (BR238), University Hospital and Faculty of Medicine, Free University, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Beelen Robert Department of Cell Biology, University Hospital and Faculty of Medicine, Free University, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Broekhoven Marjolein Department of Haematology (BR238), University Hospital and Faculty of Medicine, Free University, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Langenhuijsen Mart Department of Haematology (BR238), University Hospital and Faculty of Medicine, Free University, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- Cancer Immunology, Immunotherapy Springer-Verlag 34/6(1992-11-01), 393-398 0340-7004 34:6<393 1992 34 262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer