caa a22 4500 469074280 CHVBK 20180323132931.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF01741789 doi (NATIONALLICENCE)springer-10.1007/BF01741789 Tissue distribution of adoptively transferred adherent lymphokine-activated killer cells assessed by different cell labels [Elektronische Daten] [P. Basse, R. Herberman, M. Hokland, R. Goldfarb] Summary: Assessment of the tissue distribution of adoptively transferred adherent lymphokine-activated killer A-LAK) cells by use of51Cr indicated that these effector cells, after an initial phase in the lungs, distributed in high numbers to liver and spleen (30% and 10% of injected dose, respectively). However, when this experiment was repeated with125IdUrd as cell label, fewer than 2% and 0.5% of the injected cells distributed into liver and spleen respectively. To analyse this discrepancy, we compared the tissue distribution of51Cr- and125IdUrd-labelled A-LAK cells with that indicated by alternative direct visual methods for identification of the injected cells, such as fluorescent dyes (rhodamine and H33342) or immunohistochemical staining of asialo-GM1-positive cells. The number of i. v. injected A-LAK cells found in the liver by all visual methods ranged from 1% to 5% of the injected dose, supporting the data obtained with125IdUrd, whereas 25%-30% of the51Cr label was consistently found in this organ. Autoradiography of the liver 24 h after i. v. injection of51Cr-labelled cells revealed a background activity that was four- to fivefold higher than the control level, indicating substantial non-specific accumulation in the liver of51Cr released from A-LAK cells. We conclude that51Cr cannot be reliably used in investigations of cell traffic to the liver because of non-specific accumulation of the51Cr label, particularly in this organ. In contrast, labelling with125IdUrd or rhodamine and immunohistochemical staining of asialo-GM1-positive cells appear to be reliable and essentially equivalent methods for investigations of the fate of adoptively transferred A-LAK cells. Using these methods, we found that only few A-LAK cells redistribute to the liver upon i. v., i. e. systemic, injection, whereas 40%-50% of locally (intraportally) injected A-LAK cells remain in the liver for at least 24 h. Springer-Verlag, 1992 LAK migration nationallicence Asialo-GM1 antibody nationallicence Fluorescence nationallicence Rhodamine nationallicence Hoechst 33342 nationallicence Basse P. Pittsburgh Cancer Institute and Departments of Pathology and Medicine, University of Pittsburgh, 15 213, Pittsburgh, PA, USA aut Herberman R. Pittsburgh Cancer Institute and Departments of Pathology and Medicine, University of Pittsburgh, 15 213, Pittsburgh, PA, USA aut Hokland M. Institute of Medical Microbiology, University of Aarhus, DK-8000, Aarhus C, Denmark aut Goldfarb R. Pittsburgh Cancer Institute and Departments of Pathology and Medicine, University of Pittsburgh, 15 213, Pittsburgh, PA, USA aut Cancer Immunology, Immunotherapy Springer-Verlag 34/4(1992-07-01), 221-227 0340-7004 34:4<221 1992 34 262 https://doi.org/10.1007/BF01741789 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01741789 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Basse P. Pittsburgh Cancer Institute and Departments of Pathology and Medicine, University of Pittsburgh, 15 213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Herberman R. Pittsburgh Cancer Institute and Departments of Pathology and Medicine, University of Pittsburgh, 15 213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Hokland M. Institute of Medical Microbiology, University of Aarhus, DK-8000, Aarhus C, Denmark aut NATIONALLICENCE 700 1- Goldfarb R. Pittsburgh Cancer Institute and Departments of Pathology and Medicine, University of Pittsburgh, 15 213, Pittsburgh, PA, USA aut NATIONALLICENCE 773 0- Cancer Immunology, Immunotherapy Springer-Verlag 34/4(1992-07-01), 221-227 0340-7004 34:4<221 1992 34 262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer