caa a22 4500 469074302 CHVBK 20180323132931.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF01741795 doi (NATIONALLICENCE)springer-10.1007/BF01741795 Effect of monoclonal antibodies to early pregnancy factor (EPF) on the in vivo growth of transplantable murine tumours [Elektronische Daten] [K. Quinn, H. Morton] Summary: Neutralisation studies with monoclonal antibodies (mAbs) specific for early pregnancy factor (EPF) have shown it to be essential for the continuation of pregnancy in mice and the growth of some tumour cells in vitro. These studies report that the mAbs are also able to limit the growth of two murine tumour lines transplanted s. c. The development of MCA-2 tumours in CBA mice was unaffected by the injection of 1 mg anti-EPF IgM at the time of tumour cell inoculation. However, four doses of 500 µg anti-EPF, injected one dose per day for 4 days after tumour cell inoculation, significantly retarded tumour development such that no tumours were palpable on day 13. A similar dose regimen of control IgM had no effect on tumour size. Dose/response studies revealed that lower doses of anti-EPF administered after tumour cell inoculation were effective in retarding the growth of the MCA-2 tumours. The effect of anti-EPF mAb administration on the growth rate of palpable B16 tumours established s. c. in C57BL/6 mice was also determined. Tumours injected with 6 mg anti-EPF 5/341 or anti-EPF 5/333 mAbs showed significant decrease in the uptake of [3H]thymidine into tumour tissue, measured 16 h after injection. Furthermore, titration of sera for active EPF showed that a significant reduction in the EPF titre was associated with a significant inhibition of tumour DNA synthesis. Thus it appears that neutralisation of EPF retards tumour growth both in vitro and in vivo. In vitro the effects must be due to anti-EPF mAb interfering with a direct mechanism that contributes to the maintenance of cells in the active growing phase. However, in vivo host immunological mechanism that are modified to allow tumour survival may also be affected. The presence of EPF-induced suppressor factors curculating in the serum of tumour-bearing mice has been confirmed and the contribution of such factors to tumour progression must now be investigated. Springer-Verlag, 1992 Early pregnancy factor nationallicence EPF nationallicence Monoclonal antibodies nationallicence Tumour retardation nationallicence Quinn K. University of Queensland Department of Surgery, Royal Brisbane Hospital, 4029, Queensland, Australia aut Morton H. University of Queensland Department of Surgery, Royal Brisbane Hospital, 4029, Queensland, Australia aut Cancer Immunology, Immunotherapy Springer-Verlag 34/4(1992-07-01), 265-271 0340-7004 34:4<265 1992 34 262 https://doi.org/10.1007/BF01741795 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01741795 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Quinn K. University of Queensland Department of Surgery, Royal Brisbane Hospital, 4029, Queensland, Australia aut NATIONALLICENCE 700 1- Morton H. University of Queensland Department of Surgery, Royal Brisbane Hospital, 4029, Queensland, Australia aut NATIONALLICENCE 773 0- Cancer Immunology, Immunotherapy Springer-Verlag 34/4(1992-07-01), 265-271 0340-7004 34:4<265 1992 34 262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 469073713 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer