caa a22 4500 469074345 CHVBK 20180323132931.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF01741791 doi (NATIONALLICENCE)springer-10.1007/BF01741791 Reconstituted membranes of tumour cells (proteoliposomes) induce specific protection to murine lymphoma cells [Elektronische Daten] [Joep Bergers, Willem Den Otter, Jan De Groot, Adriana De Blois, Hub Dullens, Peter Steerenberg, Daan Crommelin] Summary: Antigens presented on cell membranes or on liposomes are usually more immunogenic than antigens in soluble form, this being one of the reasons for the weak immunogenicity of extracted tumour-associated transplantation antigens (TATA). The main objective of this study is to solubilize TATA from tumour cells and to present them on a membrane-like structure to the immune system. Crude tumour cell membranes of SL2 lymphosarcoma cells (a spontaneously arising, weakly immunogenic tumour) were solubilized with octylglucoside or sodium deoxycholate, and reconstituted membranes (proteoliposomes) were prepared by detergent removal. Mice immunized s.c. with reconstituted membranes were protected against an i. p. challenge with tumour cells. Although octylglucoside solubilized only 41% of the membrane proteins, the reconstituted membranes were as immunoprotective as crude membranes. (Glyco)proteins were probably the major membrane components in the reconstituted membranes that induce immunoprotection, as mice immunized with preparations constituted of (glyco)lipids from SL2 cells could not reject SL2 cells. If Freund's complete adjuvant was used with the first immunization injection, no potentiation of the elicited immune responses was observed. Besides the membrane TATA, SL2 cells contained an apparently non-membrane-bound TATA, which was found in the cytoplasm. It is concluded that detergent solubilization of membranes and subsequent preparation of reconstituted membranes can be used to obtain membrane tumour-associated antigens that retain activity for induction of protective tumour immunity. The major advantage of this method is that membrane proteins are solubilized and are subsequently presented on a membrane-like structure that resembles the tumour cell membrane. On theoretical and practical grounds it provides a promising alternative for whole-cell vaccines. Springer-Verlag, 1992 Tumour vaccine nationallicence Liposomes nationallicence Reconstituted membranes nationallicence Lymphoma cells nationallicence Bergers Joep Department of Pharmaceutics, Faculty of Pharmacy, University of Utrecht, P. O. Box 80.082, 3508 TB, Utrecht, The Netherlands aut Den Otter Willem Department of Pathology, University Hospital Utrecht, P. O. Box 85.500, 3508 GA, Utrecht, The Netherlands aut De Groot Jan Department of Pathology, University Hospital Utrecht, P. O. Box 85.500, 3508 GA, Utrecht, The Netherlands aut De Blois Adriana Department of Pharmaceutics, Faculty of Pharmacy, University of Utrecht, P. O. Box 80.082, 3508 TB, Utrecht, The Netherlands aut Dullens Hub Department of Pathology, University Hospital Utrecht, P. O. Box 85.500, 3508 GA, Utrecht, The Netherlands aut Steerenberg Peter Laboratory for Pathology, National Institute of Public Health and Environmental Protection, P. O. Box 1, 3720 BA, Bilthoven, The Netherlands aut Crommelin Daan Department of Pharmaceutics, Faculty of Pharmacy, University of Utrecht, P. O. Box 80.082, 3508 TB, Utrecht, The Netherlands aut Cancer Immunology, Immunotherapy Springer-Verlag 34/4(1992-07-01), 233-240 0340-7004 34:4<233 1992 34 262 https://doi.org/10.1007/BF01741791 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01741791 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bergers Joep Department of Pharmaceutics, Faculty of Pharmacy, University of Utrecht, P. O. Box 80.082, 3508 TB, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Den Otter Willem Department of Pathology, University Hospital Utrecht, P. O. Box 85.500, 3508 GA, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- De Groot Jan Department of Pathology, University Hospital Utrecht, P. O. Box 85.500, 3508 GA, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- De Blois Adriana Department of Pharmaceutics, Faculty of Pharmacy, University of Utrecht, P. O. Box 80.082, 3508 TB, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Dullens Hub Department of Pathology, University Hospital Utrecht, P. O. Box 85.500, 3508 GA, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Steerenberg Peter Laboratory for Pathology, National Institute of Public Health and Environmental Protection, P. O. Box 1, 3720 BA, Bilthoven, The Netherlands aut NATIONALLICENCE 700 1- Crommelin Daan Department of Pharmaceutics, Faculty of Pharmacy, University of Utrecht, P. O. Box 80.082, 3508 TB, Utrecht, The Netherlands aut NATIONALLICENCE 773 0- Cancer Immunology, Immunotherapy Springer-Verlag 34/4(1992-07-01), 233-240 0340-7004 34:4<233 1992 34 262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer