caa a22 4500 469074353 CHVBK 20180323132931.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF01741547 doi (NATIONALLICENCE)springer-10.1007/BF01741547 Induction of T-cell-mediated immunity against MethA fibrosarcoma by intratumoral injections of a bacillus Calmette-Guérin nucleic acid fraction [Elektronische Daten] [Etsuro Kuramoto, Osamu Yano, Shizuo Shimada] Summary: MY-1, which consists of DNA and RNA extracted and purified from bacillus Calmette-Guérin (BCG), has been shown to have strong antitumor activity against various experimental tumors. To examine the role of T cells in the antitumor mechanism of MY-1, the effect of MY-1 injection on the development of tumor-specific immunity against MethA fibrosarcoma was investigated. MY-1 injections inhibited tumor growth less effectively in T-cell-deficient nude mice than in normal BALB/c mice. MethA tumor growth was suppressed after inoculation with L3T4-positive lymphocytes from tumor-bearing mice treated with MY-1. MethA-specific delayed-type hypersensitivity was also detected in tumor-bearing mice treated with MY-1. Immunohistochemical analyses showed that many L3T4-positive and a few Lyt2-positive cells infiltrated the regressing tumors. These results indicate that intratumoral MY-1 injections induce a MethA-specific, L3T4-positive cell-mediated, delayed-type hypersensitivity, which is necessary for the tumor regression. Springer-Verlag, 1992 BCG nationallicence DNA nationallicence Antitumor immunity nationallicence Fibrosarcoma nationallicence Kuramoto Etsuro Institute of Biological Science, Mitsui Pharmaceuticals, Inc., 1900-1 Togo, 297, Mobara, Chiba, Japan aut Yano Osamu Institute of Biological Science, Mitsui Pharmaceuticals, Inc., 1900-1 Togo, 297, Mobara, Chiba, Japan aut Shimada Shizuo Institute of Biological Science, Mitsui Pharmaceuticals, Inc., 1900-1 Togo, 297, Mobara, Chiba, Japan aut Cancer Immunology, Immunotherapy Springer-Verlag 34/5(1992-09-01), 283-288 0340-7004 34:5<283 1992 34 262 https://doi.org/10.1007/BF01741547 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01741547 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuramoto Etsuro Institute of Biological Science, Mitsui Pharmaceuticals, Inc., 1900-1 Togo, 297, Mobara, Chiba, Japan aut NATIONALLICENCE 700 1- Yano Osamu Institute of Biological Science, Mitsui Pharmaceuticals, Inc., 1900-1 Togo, 297, Mobara, Chiba, Japan aut NATIONALLICENCE 700 1- Shimada Shizuo Institute of Biological Science, Mitsui Pharmaceuticals, Inc., 1900-1 Togo, 297, Mobara, Chiba, Japan aut NATIONALLICENCE 773 0- Cancer Immunology, Immunotherapy Springer-Verlag 34/5(1992-09-01), 283-288 0340-7004 34:5<283 1992 34 262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer