caa a22 4500 469074388 CHVBK 20180323132931.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF01741557 doi (NATIONALLICENCE)springer-10.1007/BF01741557 Allogeneic tumor-specific cytotoxic T lymphocytes [Elektronische Daten] [Joan Redd, Anne-Catherine Lagarde, Carol Kruse, Donald Bellgrau] Summary: We report the development of cytotoxic T lymphocytes specific for an allogeneic brain tumor in a rat model. DA strain cytotoxic T cell precursors stimulated by an allogeneic tumor (9L gliosarcoma) from the Fischer rat could generate a population of cytotoxic T lymphocytes that lysed the allogeneic 9L tumor but failed to lyse other targets, including Fischer concanavalin-A(ConA)-stimulated lymphoid blast targets. DA T cells depleted of reactivity to the Fischer haplotype (DA-f) retained reactivity to the 9L tumor, demonstrating that T cell precursors with specificity for normal Fischer alloantigens were not required for the generation of a response to the 9L Fischer tumor. The preferential lysis of the tumor target did not simply reflect a higher density of Fischer target antigens on the tumor than that found on normal Fischer ConA blast targets. First, the relative densities of class I antigen on the 9L tumor and normal Fischer ConA blasts were comparable. Second, cytotoxic T cells could not be generated from DA-f precursors when Fischer ConA blasts were used as stimulators. If DA-f T cells were simply responding to the higher density of Fischer antigen found on 9L tumor, it would have been expected that the ConA blasts expressing comparable levels of antigen to that found on the tumor would have generated cytotoxicity for both the 9L and ConA targets. We conclude that the cytotoxic T cells are specific for a determinant expressed only by the tumor. Such tumor-specific cytotoxic T cells could be useful in vivo for adoptive immunotherapy of brain tumors. Springer-Verlag, 1992 Immunotherapy nationallicence Glioma nationallicence Activated lymphocytes nationallicence Redd Joan The Barbara Davis Center for Childhood Diabetes and the Department of Microbiology/Immunology, University of Colorado Health Sciences Center, Box B 140, 80 262, Denver, CO, USA aut Lagarde Anne-Catherine The Barbara Davis Center for Childhood Diabetes and the Department of Microbiology/Immunology, University of Colorado Health Sciences Center, Box B 140, 80 262, Denver, CO, USA aut Kruse Carol Departments of Surgery and Pathology, University of Colorado Health Sciences Center, 80 262, Denver, CO, USA aut Bellgrau Donald The Barbara Davis Center for Childhood Diabetes and the Department of Microbiology/Immunology, University of Colorado Health Sciences Center, Box B 140, 80 262, Denver, CO, USA aut Cancer Immunology, Immunotherapy Springer-Verlag 34/5(1992-09-01), 349-354 0340-7004 34:5<349 1992 34 262 https://doi.org/10.1007/BF01741557 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01741557 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Redd Joan The Barbara Davis Center for Childhood Diabetes and the Department of Microbiology/Immunology, University of Colorado Health Sciences Center, Box B 140, 80 262, Denver, CO, USA aut NATIONALLICENCE 700 1- Lagarde Anne-Catherine The Barbara Davis Center for Childhood Diabetes and the Department of Microbiology/Immunology, University of Colorado Health Sciences Center, Box B 140, 80 262, Denver, CO, USA aut NATIONALLICENCE 700 1- Kruse Carol Departments of Surgery and Pathology, University of Colorado Health Sciences Center, 80 262, Denver, CO, USA aut NATIONALLICENCE 700 1- Bellgrau Donald The Barbara Davis Center for Childhood Diabetes and the Department of Microbiology/Immunology, University of Colorado Health Sciences Center, Box B 140, 80 262, Denver, CO, USA aut NATIONALLICENCE 773 0- Cancer Immunology, Immunotherapy Springer-Verlag 34/5(1992-09-01), 349-354 0340-7004 34:5<349 1992 34 262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer