caa a22 4500 469076135 CHVBK 20180323132935.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF02927873 doi (NATIONALLICENCE)springer-10.1007/BF02927873 Jayaraman R. School of Biological Sciences, Madurai Kamaraj University, 625021, Madurai, India aut Cairnsian mutagenesis in Escherichia coli [Elektronische Daten] Genetic evidence for two pathways regulated by mut S and mut L genes [R. Jayaraman] The phenomenon of Cairnsian mutagenesis was studied inEscherichia coli mutants bearing mutations inmutS,mutL,recA andlexA genes. It is shown that development of resistance to exogenous valine could be used as an example of Cairnsian response. Strains defective inmutS andmutL show a high frequency of Cairnsian mutagenesis to valine resistance. The response inmutS mutants is dependent upon cleavability of the LexA protein whereas that inmutL is not. The latter is independent ofrecA also. The need for LexA protein cleavage inmutS mutants can be bypassed by over-production of the RecA protein due to arecA operator constitutive mutation. Genetic evidence is presented to show that the products ofmutS andmutL genes negativelycontrol two pathways of Cairnsian mutagenesis. Cairnsian response is also elicited whenmutS ormutL strains are grown under conditions wherein a required nutrient is present in sub-optimal concentrations. Random, unselected mutagenic events are likely to occur during or after Cairnsian mutagenesis provided the cells are SOS inducible. Indian Academy of Sciences, 1992 Mutagenesis nationallicence Cairnsian response nationallicence mut S nationallicence mut L nationallicence SOS response nationallicence adaptive mutagenesis nationallicence Journal of Genetics Springer India 71/1-2(1992-08-01), 23-41 0022-1333 71:1-2<23 1992 71 12041 https://doi.org/10.1007/BF02927873 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02927873 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Jayaraman R. School of Biological Sciences, Madurai Kamaraj University, 625021, Madurai, India aut NATIONALLICENCE 773 0- Journal of Genetics Springer India 71/1-2(1992-08-01), 23-41 0022-1333 71:1-2<23 1992 71 12041 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer