caa a22 4500 469077743 CHVBK 20180323132939.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00167572 doi (NATIONALLICENCE)springer-10.1007/BF00167572 Identification of a novel high affinity adenosine binding protein from bovine striatum [Elektronische Daten] [Anna Lorenzen, Sigrid Grün, Heidrun Vogt, Ulrich Schwabe] Summary: In solubilized extracts from bovine striatal membranes three different binding sites for 5′-N-ethylcarboxamidoadenosine ([3H]NECA) were observed after separation of the extract by gel filtration on Sepharose CL-6B. The first peak was eluted in the void volume and contained the AZ adenosine receptor. In the second peak, [3H]NECA binding sites were eluted with a pharmacological profile characteristic of adenotin, a low affinity non-receptor adenosine binding protein. The third peak represented approximately 50% of the [3H]NECA binding activity. This site bound [3H]NECA in a reversible and saturable manner with K D of 17 nmol/l and a binding capacity of 11.3 pmol/mg protein. In competition experiments, adenosine, NECA, NAD, nnosine, 5′-AMP and S-adenosyl-L-homocysteine were the most potent ligands. In contrast to adenosine receptors, this site did neither bind adenosine receptor antagonists nor the A2 selective agonist CGS 21,680 (2-[p-(2-carboxyethyl)phenethylamino]5′-N-ethylcarboxamidoadenosine). These results suggest the existence of a novel high affinity binding site for adenosine of unknown function in bovine striatum. Springer-Verlag, 1992 Adenosine binding protein nationallicence Adenosine receptor nationallicence Bovine striatum nationallicence Radioligand binding nationallicence AMPPCP : β,γ-methyleneadenosine-5′-triphosphonate nationallicence CCPA : 2-chloro-N6-cyclopentyladenosine nationallicence CHAPS : 3-[3-(cholamidopropyl)dimethylammonio]-1-propanesulfonate nationallicence CGS 21,680 : 2-[p-(2-carboxyethyl)phenethylamino]-5′-N-ethylcarbox-amidoadenosine nationallicence CPA : N6-cyclopentyladenosine nationallicence DPCPX : 1,3-dipropyl-8-cyclopentylxanthine nationallicence GppNHp : guanosine-5′-[β,γ-imido]triphosphate nationallicence GTP[S] : guanosine-5′-O-(3-thiotriphosphate) nationallicence NBTI : S-4-nitrobenzyl-6-thioinosine nationallicence NECA : 5′-N-ethylcarbox-amidoadenosine nationallicence NECG : 5′-N-ethylcarboxamidoguanosine nationallicence PIA : N6-phenylisopropyladenosine nationallicence SAH : S-adenosyl-L-homocysteine nationallicence XAC : 8-{4-[([{(2-aminoethyl)-amino}carbonyl]-methyl)oxy]-phenyl}-1,3-dipropylxanthine nationallicence Lorenzen Anna Pharmakologisches Institut der Universität Heidelberg, Im Neuenheimer Feld 366, W-6900, Heidelberg, Federal Republic of Germany aut Grün Sigrid Pharmakologisches Institut der Universität Heidelberg, Im Neuenheimer Feld 366, W-6900, Heidelberg, Federal Republic of Germany aut Vogt Heidrun Pharmakologisches Institut der Universität Heidelberg, Im Neuenheimer Feld 366, W-6900, Heidelberg, Federal Republic of Germany aut Schwabe Ulrich Pharmakologisches Institut der Universität Heidelberg, Im Neuenheimer Feld 366, W-6900, Heidelberg, Federal Republic of Germany aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/1(1992-07-01), 63-68 0028-1298 346:1<63 1992 346 210 https://doi.org/10.1007/BF00167572 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00167572 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lorenzen Anna Pharmakologisches Institut der Universität Heidelberg, Im Neuenheimer Feld 366, W-6900, Heidelberg, Federal Republic of Germany aut NATIONALLICENCE 700 1- Grün Sigrid Pharmakologisches Institut der Universität Heidelberg, Im Neuenheimer Feld 366, W-6900, Heidelberg, Federal Republic of Germany aut NATIONALLICENCE 700 1- Vogt Heidrun Pharmakologisches Institut der Universität Heidelberg, Im Neuenheimer Feld 366, W-6900, Heidelberg, Federal Republic of Germany aut NATIONALLICENCE 700 1- Schwabe Ulrich Pharmakologisches Institut der Universität Heidelberg, Im Neuenheimer Feld 366, W-6900, Heidelberg, Federal Republic of Germany aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/1(1992-07-01), 63-68 0028-1298 346:1<63 1992 346 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer