caa a22 4500 469077751 CHVBK 20180323132940.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00167562 doi (NATIONALLICENCE)springer-10.1007/BF00167562 Release of endogenous acetylcholine in the hypothalamus of conscious rats [Elektronische Daten] [H. Prast, A. Philippu] Summary: The release of endogenous acetylcholine was investigated by the push-pull technique. The posterior hypothalamus of conscious rats was superfused through a push-pull cannula with artificial cerebrospinal fluid (ACSF) which contained 1 μmol/l neostigmine. Acetylcholine was determined in the superfusate by high pressure liquid chromatography and electrochemical detection. Hypothalamic superfusion with potassium-rich (100 mmol/l) ACSF led to a pronounced increase in the release rate of acetylcholine. Tetrodotoxin (1 μmol/l) almost abolished the basal release of the neurotransmitter. Superfusion of the hypothalamus with atropine (10 or 50 μmol/l) led to a concentration-dependent increase, whereas superfusion with oxotremorine (50 μmol/l) inhibited the release rate of acetylcholine. It is concluded that acetylcholine released into the superfusate of the hypothalamus originates from cholinergic neurons. Furthermore, the release of acetylcholine seems to be modulated by muscarinic acetylcholine receptors, probably located on cholinergic neurons of the hypothalamus. Springer-Verlag, 1992 Acetylcholine release nationallicence Rat hypothalamus nationallicence Atropine nationallicence Oxotremorine nationallicence Push-pull cannula nationallicence Prast H. Institut für Pharmakologie und Toxikologie der Universität Innsbruck, Peter-Mayr-Strasse 1, A-6020, Innsbruck, Austria aut Philippu A. Institut für Pharmakologie und Toxikologie der Universität Innsbruck, Peter-Mayr-Strasse 1, A-6020, Innsbruck, Austria aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/1(1992-07-01), 1-3 0028-1298 346:1<1 1992 346 210 https://doi.org/10.1007/BF00167562 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00167562 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Prast H. Institut für Pharmakologie und Toxikologie der Universität Innsbruck, Peter-Mayr-Strasse 1, A-6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Philippu A. Institut für Pharmakologie und Toxikologie der Universität Innsbruck, Peter-Mayr-Strasse 1, A-6020, Innsbruck, Austria aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/1(1992-07-01), 1-3 0028-1298 346:1<1 1992 346 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer