caa a22 4500 46907776X CHVBK 20180323132940.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00167573 doi (NATIONALLICENCE)springer-10.1007/BF00167573 Naloxone-reversible effect of spantide on the spinally mediated behavioural response induced by neurokinin-2 and -3 receptor agonists [Elektronische Daten] [T. Sakurada, Y. Manome, K. Katsumata, H. Uchiumi, K. Tan-No, S. Sakurada, K. Kisara] Summary: [d-Arg1, dTrp7,9, Leu11]-substance P (spantide) was tested for antagonism against the licking, biting and scratching response induced by various neurokinin (NK) receptor agonists and bombesin (Bom) in mice. When co-administered with substance P (SP) intrathecally, spantide reduced the SP-induced behavioural responses in a dose-dependent manner. The duration of this antagonistic effect was approximately 30 min. Behavioural responses induced by physalaemin (Phy), [pGlu6, l-Pro9]-SP (6-11) (septide), [pGlu6, d-Pro7]-SP (6-11) (d-septide) and eledoisin (Ele) were also dose-dependently decreased by relatively small doses of spantide. Higher doses of spantide were needed to reduce the behavioural responses induced by [Sar9, Met (O2)11]-SP, neurokinin A (NK A) and neurokinin B (NK B). No significant effect of spantide was observed against the behavioural responses elicited by Bom. Pretreatment with naloxone, an opioid antagonist, resulted in a reversible effect on the behavioural reduction of NK-2 and NK-3 receptor agonists produced by spantide. However, the effect of spantide on the NK-1 receptor agonist-induced response was unchanged by naloxone. In homogenates of mouse spinal cord, competition studies confirmed that the binding of the opioid ligand [3H]naloxone was displaced by spantide with a low but measurable affinity. These results suggest that the behavioural response to NK-2 and NK-3 receptor agonists may be partially inhibited by spantide through the activation of opioid system in the mouse spinal cord. Springer-Verlag, 1992 Spantide nationallicence Neurokinin receptor agonists nationallicence Intrathecal injection nationallicence Licking, biting and scratching nationallicence Naloxone nationallicence Opioid receptor nationallicence Sakurada T. Department of Pharmacology, Tohoku College of Pharmacy, 4-4-1 Komatsushima, 981, Aoba-ku, Sendai, Japan aut Manome Y. Department of Pharmacology, Tohoku College of Pharmacy, 4-4-1 Komatsushima, 981, Aoba-ku, Sendai, Japan aut Katsumata K. Department of Pharmacology, Tohoku College of Pharmacy, 4-4-1 Komatsushima, 981, Aoba-ku, Sendai, Japan aut Uchiumi H. Department of Pharmacology, Tohoku College of Pharmacy, 4-4-1 Komatsushima, 981, Aoba-ku, Sendai, Japan aut Tan-No K. Department of Pharmacology, Tohoku College of Pharmacy, 4-4-1 Komatsushima, 981, Aoba-ku, Sendai, Japan aut Sakurada S. Department of Pharmacology, Tohoku College of Pharmacy, 4-4-1 Komatsushima, 981, Aoba-ku, Sendai, Japan aut Kisara K. Department of Pharmacology, Tohoku College of Pharmacy, 4-4-1 Komatsushima, 981, Aoba-ku, Sendai, Japan aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/1(1992-07-01), 69-75 0028-1298 346:1<69 1992 346 210 https://doi.org/10.1007/BF00167573 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00167573 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sakurada T. Department of Pharmacology, Tohoku College of Pharmacy, 4-4-1 Komatsushima, 981, Aoba-ku, Sendai, Japan aut NATIONALLICENCE 700 1- Manome Y. Department of Pharmacology, Tohoku College of Pharmacy, 4-4-1 Komatsushima, 981, Aoba-ku, Sendai, Japan aut NATIONALLICENCE 700 1- Katsumata K. Department of Pharmacology, Tohoku College of Pharmacy, 4-4-1 Komatsushima, 981, Aoba-ku, Sendai, Japan aut NATIONALLICENCE 700 1- Uchiumi H. Department of Pharmacology, Tohoku College of Pharmacy, 4-4-1 Komatsushima, 981, Aoba-ku, Sendai, Japan aut NATIONALLICENCE 700 1- Tan-No K. Department of Pharmacology, Tohoku College of Pharmacy, 4-4-1 Komatsushima, 981, Aoba-ku, Sendai, Japan aut NATIONALLICENCE 700 1- Sakurada S. Department of Pharmacology, Tohoku College of Pharmacy, 4-4-1 Komatsushima, 981, Aoba-ku, Sendai, Japan aut NATIONALLICENCE 700 1- Kisara K. Department of Pharmacology, Tohoku College of Pharmacy, 4-4-1 Komatsushima, 981, Aoba-ku, Sendai, Japan aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/1(1992-07-01), 69-75 0028-1298 346:1<69 1992 346 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer