caa a22 4500 469077859 CHVBK 20180323132940.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00167570 doi (NATIONALLICENCE)springer-10.1007/BF00167570 Calcium mobilization and its desensitization induced by endothelins and sarafotoxin in human astrocytoma cells (1321N1): Comparison of histamine-induced calcium mobilization [Elektronische Daten] [Kazuhiko Yanai, Kazutaka Maeyama, Norimichi Nakahata, Hironori Nakanishi, Takehiko Watanabe] Summary: Intracellular free Ca2+ concentration ([Ca2+]i) was monitored in monolayer of 1321 N1 astrocytoma cells by using a fluorescent Ca2+ indicator fura-2. Endothelin-1 (ET-1), endothelin-2 (ET-2), and sarafotoxin Sb6 (SRTX) increased [Ca2+]i from 56 ± 6 nM to 360 ± 82, 120 ± 51, 143 ± 29 nM, respectively, immediately after their addition to the perfusate with maximum response of more than 0.1 μM of peptides. Endothelin-3 (< 1 μM) did not affect [Ca2+]i. The increase in [Ca2+]i in response to either ET-1, ET-2, or SRTX could be almost completely inhibited by pretreating cells with ET-1 or ET-2. The homologous desensitization of [Ca2+]i induced by ETs and SRTX is in good agreement with their affinity toward an ETA receptors. The responses in [Ca2+]i by ETs and SRTX were not affected in the desensitized state induced by the pretreatment of histamine in the presence of extracellular Ca2+. However, the response in [Ca2+]i by ETs and SRTX were reduced in the desensitized state induced by pretreatment of histamine in the absence of extracellular Ca2+. These results indicate that depletion of the intracellular Ca2+ stores is responsible for the heterologous desensitization between ETs and histamine. Springer-Verlag, 1992 Endothelins nationallicence Intracellular Ca2+ nationallicence Desensitization nationallicence Histamine nationallicence Astrocytoma cell line nationallicence Yanai Kazuhiko Department of Pharmacology I, Tohoku University School of Medicine, Seiryo-machi 2-1, 980, Aoba-Ku, Sendai, Japan aut Maeyama Kazutaka Department of Pharmacology I, Tohoku University School of Medicine, Seiryo-machi 2-1, 980, Aoba-Ku, Sendai, Japan aut Nakahata Norimichi Department of Pharmacology, Fukushima Medical College, Hikarigaoka 1, 960-12, Fukushima, Japan aut Nakanishi Hironori Department of Pharmacology, Fukushima Medical College, Hikarigaoka 1, 960-12, Fukushima, Japan aut Watanabe Takehiko Department of Pharmacology I, Tohoku University School of Medicine, Seiryo-machi 2-1, 980, Aoba-Ku, Sendai, Japan aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/1(1992-07-01), 51-56 0028-1298 346:1<51 1992 346 210 https://doi.org/10.1007/BF00167570 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00167570 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yanai Kazuhiko Department of Pharmacology I, Tohoku University School of Medicine, Seiryo-machi 2-1, 980, Aoba-Ku, Sendai, Japan aut NATIONALLICENCE 700 1- Maeyama Kazutaka Department of Pharmacology I, Tohoku University School of Medicine, Seiryo-machi 2-1, 980, Aoba-Ku, Sendai, Japan aut NATIONALLICENCE 700 1- Nakahata Norimichi Department of Pharmacology, Fukushima Medical College, Hikarigaoka 1, 960-12, Fukushima, Japan aut NATIONALLICENCE 700 1- Nakanishi Hironori Department of Pharmacology, Fukushima Medical College, Hikarigaoka 1, 960-12, Fukushima, Japan aut NATIONALLICENCE 700 1- Watanabe Takehiko Department of Pharmacology I, Tohoku University School of Medicine, Seiryo-machi 2-1, 980, Aoba-Ku, Sendai, Japan aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/1(1992-07-01), 51-56 0028-1298 346:1<51 1992 346 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer