caa a22 4500 469077875 CHVBK 20180323132940.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00167574 doi (NATIONALLICENCE)springer-10.1007/BF00167574 Veratridine causes the Ca2+-dependent increase in diacylglycerol formation and translocation of protein kinase C to membranes in cultured bovine adrenal medullary cells [Elektronische Daten] [Yasuhito Uezono, Akihiko Wada, Nobuyuki Yanagihara, Hideyuki Kobayashi, Tsunetaka Mizuki, Takeshi Terao, Yoshiro Koda, Futoshi Izumi] Summary: Our previous studies suggested that protein kinase C is involved in the veratridine (an activator of voltage-dependent Na+ channels)-induced phosphorylation and activation of tyrosine hydroxylase as well as the synthesis of catecholamines in adrenal medulla (Uezono et al. 1989). In the present study, we investigated whether treatment of cultured bovine adrenal medullary cells with veratridine causes the accumulation of diacylglycerol, a physiological activator of protein kinase C and the translocation of protein kinase C from cytosol to membrane, a process required for protein kinase C activation. Veratridine (100 μmol/l) increased diacylglycerol level about 2.2 fold in a monophasic manner, with peaking at 5 min and declining toward the basal level within 20 min. Veratridine also increased membrane protein kinase C from 15.6% to 26.9% of total protein kinase C in a timecourse similar to that of diacylglycerol accumulation. Both stimulatory effects of veratridine were inhibited by tetrodotoxin and not observed in Ca2+-free, EGTA-containing medium. Amiloride, an inhibitor of Na+/Ca2+ and Na+/H+ exchange, did not alter veratridine-induced events. These results suggest that veratridine-induced Ca2+ influx contributes to the accumulation of diacylglycerol and the activation of protein kinase C in adrenal medullary cells. Springer-Verlag, 1992 Amiloride nationallicence Calcium nationallicence Diacylglycerol nationallicence Protein kinase C nationallicence Veratridine nationallicence Uezono Yasuhito Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, 807, Yahatanishi-ku, Kitakyushu, Japan aut Wada Akihiko Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, 807, Yahatanishi-ku, Kitakyushu, Japan aut Yanagihara Nobuyuki Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, 807, Yahatanishi-ku, Kitakyushu, Japan aut Kobayashi Hideyuki Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, 807, Yahatanishi-ku, Kitakyushu, Japan aut Mizuki Tsunetaka Department of 2nd Internal Medicine, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, 807, Yahatanishi-ku, Kitakyushu, Japan aut Terao Takeshi Department of Psychiatry, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, 807, Yahatanishi-ku, Kitakyushu, Japan aut Koda Yoshiro Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, 807, Yahatanishi-ku, Kitakyushu, Japan aut Izumi Futoshi Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, 807, Yahatanishi-ku, Kitakyushu, Japan aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/1(1992-07-01), 76-81 0028-1298 346:1<76 1992 346 210 https://doi.org/10.1007/BF00167574 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00167574 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Uezono Yasuhito Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, 807, Yahatanishi-ku, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Wada Akihiko Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, 807, Yahatanishi-ku, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Yanagihara Nobuyuki Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, 807, Yahatanishi-ku, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Kobayashi Hideyuki Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, 807, Yahatanishi-ku, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Mizuki Tsunetaka Department of 2nd Internal Medicine, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, 807, Yahatanishi-ku, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Terao Takeshi Department of Psychiatry, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, 807, Yahatanishi-ku, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Koda Yoshiro Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, 807, Yahatanishi-ku, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Izumi Futoshi Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, 807, Yahatanishi-ku, Kitakyushu, Japan aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/1(1992-07-01), 76-81 0028-1298 346:1<76 1992 346 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer