caa a22 4500 469077905 CHVBK 20180323132940.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00167575 doi (NATIONALLICENCE)springer-10.1007/BF00167575 Electrophysiologic and inotropic effects of α-adrenoceptor stimulation in human isolated atrial heart muscle [Elektronische Daten] [Ulrich Jahnel, Helga Jakob, Hermann Nawrath] Summary: The effects of α-adrenoceptor stimulation on force of contraction were investigated in human atrial heart muscle and compared with those of β-adrenoceptor stimulation. The maximal positive inotropic effect produced by stimulation of α-adrenoceptors with phenylephrine (in the presence of atenolol 10 μmol/l) was significantly smaller than that seen in response to β-adrenoceptor stimulation with isoprenaline. The maximal effect of phenylephrine (25% of the maximal effect of isoprenaline) required far higher concentrations (1 mmol/l) than isoprenaline (100 nmol/l); the EC50 values amounted to 33.1 μmol/l and 3.3 nmol/l, respectively. In the presence of the α-adrenoceptor blocking agent phentolamine (1 μmol/l), the concentration-response curve of phenylephrine was displaced to higher concentrations of the agonist; under these conditions, the EC50 value amounted to 52.5 μmol/l, The effects of the catecholamines noradrenaline and adrenaline on force of contraction remained unchanged in the presence of phentolamine (1 μmol/l), or prazosin (1 μmol/l), The positive inotropic effect of phenylephrine (1 mmol/l) was associated with a slight decrease in action potential duration; the effects on action potential were completely blocked in the presence of phentolamine (1 μmol/l) These findings support the view that selective stimulation of α-adrenoceptors may mediate a small but detectable positive inotropic effect in human atrial tissue under in vitro conditions. The requirement of high concentrations of α-adrenoceptor agonists and the lack of effects of the endogenous catecholamines adrenaline and noradrenaline on α-adrenoceptors (in concentrations which fully elicit the β-adrenoceptors-mediated response) do not provide a basis for a functional role of α-adrenoceptor-mediated effects under in vivo conditions. It is more likely that adrenaline- or noradrenaline-mediated changes in the force of contraction in the human atrium are virtually exclusively due to the stimulation of β-adrenoceptors. Springer-Verlag, 1992 Human atrium nationallicence Action potential nationallicence Force of contraction nationallicence Phenylephrine nationallicence Noradrenaline nationallicence Isoprenaline nationallicence Jahnel Ulrich Pharmakologisches Institut der Universität Mainz, Obere Zahlbacher Strasse 67, W-6500, Mainz, Federal Republic of Germany aut Jakob Helga Pharmakologisches Institut der Universität Mainz, Obere Zahlbacher Strasse 67, W-6500, Mainz, Federal Republic of Germany aut Nawrath Hermann Pharmakologisches Institut der Universität Mainz, Obere Zahlbacher Strasse 67, W-6500, Mainz, Federal Republic of Germany aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/1(1992-07-01), 82-87 0028-1298 346:1<82 1992 346 210 https://doi.org/10.1007/BF00167575 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00167575 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jahnel Ulrich Pharmakologisches Institut der Universität Mainz, Obere Zahlbacher Strasse 67, W-6500, Mainz, Federal Republic of Germany aut NATIONALLICENCE 700 1- Jakob Helga Pharmakologisches Institut der Universität Mainz, Obere Zahlbacher Strasse 67, W-6500, Mainz, Federal Republic of Germany aut NATIONALLICENCE 700 1- Nawrath Hermann Pharmakologisches Institut der Universität Mainz, Obere Zahlbacher Strasse 67, W-6500, Mainz, Federal Republic of Germany aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/1(1992-07-01), 82-87 0028-1298 346:1<82 1992 346 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer