caa a22 4500 469078014 CHVBK 20180323132940.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00168737 doi (NATIONALLICENCE)springer-10.1007/BF00168737 Direct demonstration of dopamine D1-like receptor sites in the ciliary body of the rabbit eye by light microscope autoradiography [Elektronische Daten] [Raffaele Mancino, Luciano Cerulli, Alberto Ricci, Francesco Amenta] Summary: The pharmacological characteristics and the anatomical localization of [3H]-SCH 23390 in sections of the ciliary body of the rabbit eye were analyzed using a radioreceptor assay and autoradiographic techniques. [3H]-SCH 23390 was bound to sections of rabbit ciliary body in a manner consistent with the labelling of D1-like receptor sites. The dissociation constant (K d) was 0.62 nmol/l, while the maximum binding capacity (Bmax) was 117 ± 9 fmol/mg tissue. Light microscope autoradiography revealed [3H]-SCH 23390 binding sites within the epithelium of the ciliary processes, which is the ocular structure involved in the secretion of aqueous humor. No specific accumulation of silver grains was noticeable within the iridocorneal angle, which is the structure involved in the outflow of aqueous humor. These findings suggest that the rise in intraocular pressure caused by D1 receptor agonists is probably mediated by an increase of aqueous humor formation rather than by an inhibition of the outflow of aqueous humor. Springer-Verlag, 1992 Ciliary body nationallicence Ciliary epithelium nationallicence Dopamine D1 receptors nationallicence Autoradiography nationallicence Rabbit nationallicence Mancino Raffaele Cattedra di Ottica Fisiopatologica, Dipartimento di Chirurgia, University "Tor Vergata”, Roma, Italy aut Cerulli Luciano Cattedra di Ottica Fisiopatologica, Dipartimento di Chirurgia, University "Tor Vergata”, Roma, Italy aut Ricci Alberto Dipartimento di Scienze Cardiovascolari e Respiratorie, University "La Sapienza”, Roma, Italy aut Amenta Francesco Lab. Neuromorfologia e Neuropatologia, Dipartimento di Sanitá Pubblica e Biologia Cellulare, Universitá "Tor Vergata”, Roma, Italy aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/6(1992-12-01), 644-648 0028-1298 346:6<644 1992 346 210 https://doi.org/10.1007/BF00168737 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00168737 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mancino Raffaele Cattedra di Ottica Fisiopatologica, Dipartimento di Chirurgia, University "Tor Vergata”, Roma, Italy aut NATIONALLICENCE 700 1- Cerulli Luciano Cattedra di Ottica Fisiopatologica, Dipartimento di Chirurgia, University "Tor Vergata”, Roma, Italy aut NATIONALLICENCE 700 1- Ricci Alberto Dipartimento di Scienze Cardiovascolari e Respiratorie, University "La Sapienza”, Roma, Italy aut NATIONALLICENCE 700 1- Amenta Francesco Lab. Neuromorfologia e Neuropatologia, Dipartimento di Sanitá Pubblica e Biologia Cellulare, Universitá "Tor Vergata”, Roma, Italy aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/6(1992-12-01), 644-648 0028-1298 346:6<644 1992 346 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer