caa a22 4500 469078413 CHVBK 20180323132942.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00171079 doi (NATIONALLICENCE)springer-10.1007/BF00171079 Evidence for M1 muscarinic cholinoceptors mediating facilitation of noradrenaline release in guinea-pig carotid artery [Elektronische Daten] [Miguel Casado, Jesús Marin, Mercedes Salaices] Summary: The muscarinic agonists acetylcholine (150 μmol/l), carbachol (1-10 μmol/l) and McN-A-343 (1-50 μmol/l, selective for M1 receptors) increased, in a concentration-dependent manner, the electrically-evoked tritium overflow from guinea-pig carotid arteries preincubated with [3H]-noradrenaline. The increase caused by acetylcholine was not modified by hexamethonium (300 μmol/l) but was reduced by the muscarinic receptor antagonists methylatropinium (0.5 and 1 nmol/l, nonselective), pirenzepine (1 and 5 μmol/l, M1-selective), methoctramine (1 and 5 μmol/l, M2-selective) and pfluoro-hexahydro-sila-difenidol (0.1-1 μmol/l, M3-selective). The order of potencies (expressed as negative logarithms of concentrations that reduced by 50% the facilitatory effect of acetylcholine) was: methylatropinium (9.93) > pirenzepine (8.83) > p-fluoro-hexahydro-siladifenidol (6.81) ≥ methoctramine (6.20). These results demonstrate the existence of facilitatory M1 receptors modulating noradrenaline release in blood vessels. Springer-Verlag, 1991 Facilitatory muscarinic acetylcholine receptor nationallicence Noradrenaline release nationallicence Guinea-pig carotid artery nationallicence Casado Miguel Departamento de Farmacología y Terapéutica, Facultad de Medicina, Universidad Autónoma, C/Arzobispo Morcillo, E-4, 28029, Madrid, Spain aut Marin Jesús Departamento de Farmacología y Terapéutica, Facultad de Medicina, Universidad Autónoma, C/Arzobispo Morcillo, E-4, 28029, Madrid, Spain aut Salaices Mercedes Departamento de Farmacología y Terapéutica, Facultad de Medicina, Universidad Autónoma, C/Arzobispo Morcillo, E-4, 28029, Madrid, Spain aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/4(1992-10-01), 391-394 0028-1298 346:4<391 1992 346 210 https://doi.org/10.1007/BF00171079 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00171079 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Casado Miguel Departamento de Farmacología y Terapéutica, Facultad de Medicina, Universidad Autónoma, C/Arzobispo Morcillo, E-4, 28029, Madrid, Spain aut NATIONALLICENCE 700 1- Marin Jesús Departamento de Farmacología y Terapéutica, Facultad de Medicina, Universidad Autónoma, C/Arzobispo Morcillo, E-4, 28029, Madrid, Spain aut NATIONALLICENCE 700 1- Salaices Mercedes Departamento de Farmacología y Terapéutica, Facultad de Medicina, Universidad Autónoma, C/Arzobispo Morcillo, E-4, 28029, Madrid, Spain aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/4(1992-10-01), 391-394 0028-1298 346:4<391 1992 346 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer