caa a22 4500 469078464 CHVBK 20180323132942.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00171080 doi (NATIONALLICENCE)springer-10.1007/BF00171080 Nicotinic and muscarinic components of rat brain dopamine synthesis stimulation induced by physostigmine [Elektronische Daten] [Johan Grenhoff, Torgny Svensson] Summary: The role of a putative cholinergic control of ascending midbrain dopamine neurons was studied with biochemical methods in the unanaesthetized male albino rat. Post-mortem catechols were measured with high performance liquid chromatography with electrochemical detection. The acety1cholinesterase inhibitor physostigmine (0.5 mg/kg s.c.) enhanced l-dihydroxyphenylalanine (DOPA) and 3,4-dihydroxyphenylacetic acid (DOPAC) levels in both the corpus striatum and limbic areas (nucleus accumbens) after inhibition of aromatic amino acid decarboxylase with NSD-1015, indicating an enhanced synthesis of dopamine in these brain regions. The effect of physostigmine was blocked both in the corpus striatum and in limbic areas by the centrally penetrating muscarinic antagonist scopolamine (1.0 mg/kg s.c.). In contrast, the nicotinic antagonist mecamylamine (1.0 mg/kg s. c.) significantly reduced the stimulatory effect of physostigmine in limbic areas, but not in the corpus striatum. The present results suggest that ascending dopamine neurons are influenced by cholinergic synaptic transmission being mediated mainly by muscarinic receptors as regards the nigrostriatal system, and by both nicotinic and muscarinic receptors as regards the mesolimbic system. The nicotinic influence appears to primarily control phasic activity of the dopamine neurons. Springer-Verlag, 1992 Limbic system nationallicence Mecamylamine nationallicence Nicotine nationallicence Scopolamine nationallicence Striatum nationallicence Grenhoff Johan Department of Pharmacology, Karolinska Institutet, S-104 01, P.O. Box 60400, Stockholm, Sweden aut Svensson Torgny Department of Pharmacology, Karolinska Institutet, S-104 01, P.O. Box 60400, Stockholm, Sweden aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/4(1992-10-01), 395-398 0028-1298 346:4<395 1992 346 210 https://doi.org/10.1007/BF00171080 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00171080 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Grenhoff Johan Department of Pharmacology, Karolinska Institutet, S-104 01, P.O. Box 60400, Stockholm, Sweden aut NATIONALLICENCE 700 1- Svensson Torgny Department of Pharmacology, Karolinska Institutet, S-104 01, P.O. Box 60400, Stockholm, Sweden aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/4(1992-10-01), 395-398 0028-1298 346:4<395 1992 346 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer