caa a22 4500 469078545 CHVBK 20180323132942.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00169012 doi (NATIONALLICENCE)springer-10.1007/BF00169012 Photochemically induced thrombosis of the rat coronary artery and functional evaluation of thrombus formation by occurrence of ventricular arrhythmias [Elektronische Daten] Effects of acetylsalicylic acid and a thromboxan A2 synthetase inhibitor of thrombus formation [Miho Fukuchi, Toshihiko Uematsu, Sefchi Araki, Mitsuyoshi Nakashima] Summary: During i. v. infusion of rose bengal (48 mg/kg/h), the proximal portion of the rat left coronary artery was illuminated from the outside of the myocardium by green light (540 nm) to produce a transluminal thrombus subsequent to endothelial damages. The primary endothelial damages within the illuminated vascular portion, which resulted from the photochemical reaction between the dye and green light, and the subsequent formation of transluminal platelet-rich thrombus, were easily revealed by both light and electron microscopy. The establishment of the thrombus was accompanied in all cases by the occurrence of ventricular arrhythmias due to myocardial ischaemia. The times required to initiate ventricular premature beats (VPBs) and ventricular tachycardia (VT) were 381 ± 96 s and 444 ± 114 s (mean ± SEM, n = 10), respectively. Pretreatment of the rat with acetylsalicylic acid (3 and 10 mg/kg, i. v.) before the initiation of illumination had no effect on the times required to exhibit the first VPBs and VT, the incidences of both types of arrhythmias were not reduced, and the thrombus was finally formed. On the other hand, pretreatment with Y-20811, a novel thromboxane A2 synthetase inhibitor (0.3, 1 and 3 mg/kg, i. v.), delayed the onset of both VPB and VT in a dose-dependent manner. The incidences of VPBs and VT were significantly reduced at 1 and 3 mg/kg, and the thrombus formation was prevented. The formation of a transluminal thrombus in the left coronary artery by the present technique was highly reproducible and could be functionally evaluated by the occurrence of ventricular arrhythmias. Springer-Verlag, 1992 Photochemical reaction nationallicence Thrombosis nationallicence Coronary artery nationallicence Arrhythmia nationallicence Thromboxane A2 synthetase inhibitor nationallicence Fukuchi Miho Department of Pharmacology, Hamamatsu University School of Medicine, 3600 Handa-cho, 431-31, Hamamatsu, Japan aut Uematsu Toshihiko Department of Pharmacology, Hamamatsu University School of Medicine, 3600 Handa-cho, 431-31, Hamamatsu, Japan aut Araki Sefchi Department of Pharmacology, Hamamatsu University School of Medicine, 3600 Handa-cho, 431-31, Hamamatsu, Japan aut Nakashima Mitsuyoshi Department of Pharmacology, Hamamatsu University School of Medicine, 3600 Handa-cho, 431-31, Hamamatsu, Japan aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/5(1992-11-01), 550-554 0028-1298 346:5<550 1992 346 210 https://doi.org/10.1007/BF00169012 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00169012 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fukuchi Miho Department of Pharmacology, Hamamatsu University School of Medicine, 3600 Handa-cho, 431-31, Hamamatsu, Japan aut NATIONALLICENCE 700 1- Uematsu Toshihiko Department of Pharmacology, Hamamatsu University School of Medicine, 3600 Handa-cho, 431-31, Hamamatsu, Japan aut NATIONALLICENCE 700 1- Araki Sefchi Department of Pharmacology, Hamamatsu University School of Medicine, 3600 Handa-cho, 431-31, Hamamatsu, Japan aut NATIONALLICENCE 700 1- Nakashima Mitsuyoshi Department of Pharmacology, Hamamatsu University School of Medicine, 3600 Handa-cho, 431-31, Hamamatsu, Japan aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/5(1992-11-01), 550-554 0028-1298 346:5<550 1992 346 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer