caa a22 4500 469078677 CHVBK 20180323132943.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00169010 doi (NATIONALLICENCE)springer-10.1007/BF00169010 Characterization of soluble platelet guanylyl cyclase with peptide antibodies [Elektronische Daten] [Florian Guthmann, Bernd Mayer, Doris Koesling, Walter Kukovetz, Eycke Böhme] Summary: Soluble guanylyl cyclase partially purified from bovine and human platelets was characterized with antibodies raised against synthetic peptides corresponding to different sequences of the α1- and β1-subunits of the bovine lung enzyme. On immunoblots, the platelet guanylyl cyclase was recognized by the four antisera used, with the exception of an antiserum against the C-terminus of the β1-subunit which did not react with the human platelet but with the bovine platelet β1-subunit. Furthermore the human platelet β1-subunit exhibited a slightly lower molecular mass than the bovine protein. The C-terminal antibodies precipitated native platelet and lung guanylyl cyclase activity. In contrast an antibody against a peptide out of the putative catalytic domain, which is highly conserved between all guanylyl cyclases sequenced so far, did not precipitate native guanylyl cyclase, although it recognized both subunits on immunoblots, suggesting that the respective amino acid sequence is located in an inner site of the protein. Springer-Verlag, 1992 Guanylyl cyclase nationallicence Platelets nationallicence Sequence directed antibodies nationallicence Immunoprecipitation nationallicence GCpep2 : YGPEVWEDIKKEA (one letter code) nationallicence GCpep3 : SRKNTGTEETEQDEN nationallicence GCpep5 : VYKVETVGDKYMTVSGLP nationallicence GCpep8 : KKDVEEANANFLGKASGID nationallicence TBS-T : Tris-buffered saline, containing 0.0501o Tween 20 nationallicence Guthmann Florian Institut für Pharmakologie, Freie Universität Berlin, Thielallee 67-73, W-1000, Berlin 33, Federal Republic of Germany aut Mayer Bernd Institut für Pharmakologie, Freie Universität Berlin, Thielallee 67-73, W-1000, Berlin 33, Federal Republic of Germany aut Koesling Doris Institut für Pharmakologie, Freie Universität Berlin, Thielallee 67-73, W-1000, Berlin 33, Federal Republic of Germany aut Kukovetz Walter Institut für Pharmakodynamik und Toxikologie, Karl-Franzens-Universität Graz, Universitätsplatz 2, A-8010, Graz, Austria aut Böhme Eycke Institut für Pharmakologie, Freie Universität Berlin, Thielallee 67-73, W-1000, Berlin 33, Federal Republic of Germany aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/5(1992-11-01), 537-541 0028-1298 346:5<537 1992 346 210 https://doi.org/10.1007/BF00169010 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00169010 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guthmann Florian Institut für Pharmakologie, Freie Universität Berlin, Thielallee 67-73, W-1000, Berlin 33, Federal Republic of Germany aut NATIONALLICENCE 700 1- Mayer Bernd Institut für Pharmakologie, Freie Universität Berlin, Thielallee 67-73, W-1000, Berlin 33, Federal Republic of Germany aut NATIONALLICENCE 700 1- Koesling Doris Institut für Pharmakologie, Freie Universität Berlin, Thielallee 67-73, W-1000, Berlin 33, Federal Republic of Germany aut NATIONALLICENCE 700 1- Kukovetz Walter Institut für Pharmakodynamik und Toxikologie, Karl-Franzens-Universität Graz, Universitätsplatz 2, A-8010, Graz, Austria aut NATIONALLICENCE 700 1- Böhme Eycke Institut für Pharmakologie, Freie Universität Berlin, Thielallee 67-73, W-1000, Berlin 33, Federal Republic of Germany aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/5(1992-11-01), 537-541 0028-1298 346:5<537 1992 346 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer