caa a22 4500 46907874X CHVBK 20180323132943.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00165307 doi (NATIONALLICENCE)springer-10.1007/BF00165307 Reduction of postischemic leukocyte-endothelium interaction by adenosine via A2 receptor [Elektronische Daten] [Dirk Nolte, Anna Lorenzen, Hans-Anton Lehr, Franz-Josef Zimmer, Karl-Norbert Klotz, Konrad Messmer] Summary: The adhesion of leukocytes to the endothelium of postcapillary venules hallmarks a key event in ischemia-reperfusion injury. Adenosine has been shown to protect from postischemic reperfusion injury, presumably through inhibition of postischemic leukocyte-endothelial interaction. This study was performed to investigate in vivo by which receptors the effect of adenosine on postischemic leukocyte-endothelium interaction is mediated. The hamster dorsal skinfold model and fluorescence microscopy were used for intravital investigation of red cell velocity, vessel diameter, and leukocyte-endothelium interaction in postcapillary venules of a thin striated skin muscle. Leukocytes were stained in vivo with acridine orange (0.5 mg kg−1 min−1 i.v.). Parameters were assessed prior to induction of 4 h ischemia to the muscle tissue and 0.5 h, 2 h, and 24 h after reperfusion. Adenosine, the adenosine A1-selective agonist 2-chloro-N6-cyclopentyladenosine (CCPA), the A2-selective agonist CGS 21,680, the non-selective adenosine receptor antagonist xanthine amine congener (XAC), and the adenosine uptake blocker S-(p-nitrobenzyl)-6-thioinosine (NBTI) were infused via jugular vein starting 15 min prior to release of ischemia until 0.5 h after reperfusion. Adenosine and CGS 21,689 significantly reduced postischemic leukocyte-endothelium interaction 0.5 h after reperfusion (p<0.01), while no inhibitory effect was observed with CCPA. Coadministration of XAC blocked the inhibitory effects of adenosine. Infusion of NBTI alone effectively decreased postischemic leukocyte-endothelium interaction. These findings indicate that adenosine reduces postischemic leukocyte-endothelium interaction via A2 receptor and suggest a protective role of endogenous adenosine during ischemia-reperfusion. Springer-Verlag, 1992 Adenosine receptors nationallicence Ischemia/reperfusion nationallicence Leukocyte/endothelium interaction nationallicence Microcirculation nationallicence AC : adhesion coefficient nationallicence CAMAS : computer-assisted microcirculation analysis system nationallicence CCPA : 2-chloro-N6-cyclopentyladenosine nationallicence CGS21,680 : 2-[p-(2-carboxyethyl)-phenethylamino]-5′-N-ethylcarboxamido-adenosine nationallicence DMSO : dimethyl sulfoxide nationallicence NBTI : S-p-(nitrobenzyl)-6-thioinosine nationallicence RBCV : red blood cell velocity nationallicence WBCV : white blood cell velocity nationallicence XAC : xanthine amine congener nationallicence Nolte Dirk Institute for Surgical Research, University of Munich, Klinikum Großhadern, Marchioninistrasse 15, W-8000, Munich, Federal Republic of Germany aut Lorenzen Anna Institute of Pharmacology, University of Heidelberg, W-6900, Heidelberg, Federal Republic of Germany aut Lehr Hans-Anton Institute for Surgical Research, University of Munich, Klinikum Großhadern, Marchioninistrasse 15, W-8000, Munich, Federal Republic of Germany aut Zimmer Franz-Josef Institute of Pharmacology, University of Heidelberg, W-6900, Heidelberg, Federal Republic of Germany aut Klotz Karl-Norbert Institute of Pharmacology, University of Heidelberg, W-6900, Heidelberg, Federal Republic of Germany aut Messmer Konrad Institute for Surgical Research, University of Munich, Klinikum Großhadern, Marchioninistrasse 15, W-8000, Munich, Federal Republic of Germany aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/2(1992-08-01), 234-237 0028-1298 346:2<234 1992 346 210 https://doi.org/10.1007/BF00165307 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00165307 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nolte Dirk Institute for Surgical Research, University of Munich, Klinikum Großhadern, Marchioninistrasse 15, W-8000, Munich, Federal Republic of Germany aut NATIONALLICENCE 700 1- Lorenzen Anna Institute of Pharmacology, University of Heidelberg, W-6900, Heidelberg, Federal Republic of Germany aut NATIONALLICENCE 700 1- Lehr Hans-Anton Institute for Surgical Research, University of Munich, Klinikum Großhadern, Marchioninistrasse 15, W-8000, Munich, Federal Republic of Germany aut NATIONALLICENCE 700 1- Zimmer Franz-Josef Institute of Pharmacology, University of Heidelberg, W-6900, Heidelberg, Federal Republic of Germany aut NATIONALLICENCE 700 1- Klotz Karl-Norbert Institute of Pharmacology, University of Heidelberg, W-6900, Heidelberg, Federal Republic of Germany aut NATIONALLICENCE 700 1- Messmer Konrad Institute for Surgical Research, University of Munich, Klinikum Großhadern, Marchioninistrasse 15, W-8000, Munich, Federal Republic of Germany aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/2(1992-08-01), 234-237 0028-1298 346:2<234 1992 346 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer