caa a22 4500 469078774 CHVBK 20180323132943.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00165302 doi (NATIONALLICENCE)springer-10.1007/BF00165302 Characterization of the [3H]-desipramine binding site of the bovine adrenomedullary plasma membrane [Elektronische Daten] [J. Michael-Hepp, B. Blum, H. Bönisch] Summary: The specific (i.e. nisoxetine-sensitive) binding of [3H]desipramine was studied in membranes prepared from bovine adrenal medullae. (1) [3H]desipramine bound reversibly and with high affinity (K D = 2.8 nmol/l) to a single class of non-interacting binding sites (Hill coefficient = 0.96); the maximal number of binding sites (Bmax) was 2.1 pmol/mg protein. (2) Binding of [3H]desipramine was dependent on [Na+] and [Cl−]. Increasing the concentrations of these ions increased binding. (3) Substrates and inhibitors of the neuronal noradrenaline transport system (uptake,) inhibited binding of [3H]desipramine with a rank order of potency typical for an interaction with the uptake, carrier. The characteristics of [3H]desipramine binding remained essentially unchanged after solubilization of adrenomedullary membranes with the non-ionic detergent digitonin. The results indicate that the plasma membrane of bovine adreno-medulary cells is endowed with the neuronal uptake1 transporter. Springer-Verlag, 1992 Neuronal noradrenaline transporter nationallicence Uptake1 nationallicence [3H]Desipramine binding nationallicence Bovine adrenal medulla nationallicence Membrane solubilization nationallicence Michael-Hepp J. Institut für Pharmakologie and Toxikologie der Universität Wurzburg, Versbacherstrasse 9, D-8700, Wurzburg, FRG aut Blum B. Institut für Pharmakologie and Toxikologie der Universität Wurzburg, Versbacherstrasse 9, D-8700, Wurzburg, FRG aut Bönisch H. Institut für Pharmakologie and Toxikologie der Universität Bonn, Reuterstrasse 2b, D-5300, Bonn, FRG aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/2(1992-08-01), 203-207 0028-1298 346:2<203 1992 346 210 https://doi.org/10.1007/BF00165302 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00165302 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Michael-Hepp J. Institut für Pharmakologie and Toxikologie der Universität Wurzburg, Versbacherstrasse 9, D-8700, Wurzburg, FRG aut NATIONALLICENCE 700 1- Blum B. Institut für Pharmakologie and Toxikologie der Universität Wurzburg, Versbacherstrasse 9, D-8700, Wurzburg, FRG aut NATIONALLICENCE 700 1- Bönisch H. Institut für Pharmakologie and Toxikologie der Universität Bonn, Reuterstrasse 2b, D-5300, Bonn, FRG aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 346/2(1992-08-01), 203-207 0028-1298 346:2<203 1992 346 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer