caa a22 4500 469078855 CHVBK 20180323132943.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00175469 doi (NATIONALLICENCE)springer-10.1007/BF00175469 Comparison of the effects of neuropeptide K and neuropeptide γ with neurokinin A at NK2 receptors in the hamster urinary bladder [Elektronische Daten] [Paul van Giersbergen, Scott Shatzer, Elizabeth Burcher, Stephen Buck] Summary: Neuropeptide K (NPK) and neuropeptide γ (NPγ) are two endogenous N-terminally extended forms of neurokinin A (NKA). Here, we compared their effects with those of NKA on 125I-NKA binding, phosphatidyl-inositol (PI) turnover and smooth muscle contraction in the hamster urinary bladder. NPK, NPγ and NKA were equipotent in competing 125I-NKA from NK2 receptors in crude hamster bladder membranes. All three peptides stimulated PI turnover by approximately 750% with similar potency. In a third series of experiments, these peptides had similar efficacy in inducing a dose-dependent contraction of bladder smooth muscle. The NK2 receptor selective antagonist L-659,877 (cyclo[Leu-Met-Gln-Trp-Phe-Gly]) inhibited the stimulation of PI turnover and bladder contractions induced by all three tachykinins. The present results show that NKA, NPK and NPγ display a similar biological profile. The N-terminal extensions of NPK and NPγ appear not to influence binding of these peptides to NK2 receptors, NK2 receptor mediated stimulation of PI turnover, or smooth muscle contraction in hamster urinary bladder. Springer-Verlag, 1992 NK2 receptors nationallicence 125I-neurokinin A competition nationallicence Phosphatidylinositol turnover nationallicence Smooth muscle contraction nationallicence Hamster urinary bladder nationallicence van Giersbergen Paul Marion Merrell Dow Research Institute, 2110 E. Galbraith Road, 45215, Cincinnati, OH, USA aut Shatzer Scott Marion Merrell Dow Research Institute, 2110 E. Galbraith Road, 45215, Cincinnati, OH, USA aut Burcher Elizabeth School of Physiology and Pharmacology, University of New South Wales, 2033, NSW, Australia aut Buck Stephen Marion Merrell Dow Research Institute, 2110 E. Galbraith Road, 45215, Cincinnati, OH, USA aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/1(1992-01-01), 51-56 0028-1298 345:1<51 1992 345 210 https://doi.org/10.1007/BF00175469 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00175469 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van Giersbergen Paul Marion Merrell Dow Research Institute, 2110 E. Galbraith Road, 45215, Cincinnati, OH, USA aut NATIONALLICENCE 700 1- Shatzer Scott Marion Merrell Dow Research Institute, 2110 E. Galbraith Road, 45215, Cincinnati, OH, USA aut NATIONALLICENCE 700 1- Burcher Elizabeth School of Physiology and Pharmacology, University of New South Wales, 2033, NSW, Australia aut NATIONALLICENCE 700 1- Buck Stephen Marion Merrell Dow Research Institute, 2110 E. Galbraith Road, 45215, Cincinnati, OH, USA aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/1(1992-01-01), 51-56 0028-1298 345:1<51 1992 345 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer