caa a22 4500 46907891X CHVBK 20180323132943.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00175475 doi (NATIONALLICENCE)springer-10.1007/BF00175475 Effect of 3-morpholinosydnonimine (SIN-1) and NG-nitro- l -arginine (NNA) on isolated perfused anaphylactic guinea-pig hearts [Elektronische Daten] [K. Thelen, A. Dembinska-Kiéc, D. Pallapies, Th. Simmet, B. Peskar] Summary: The modulating effects of exogenous and endogenous nitric oxide (NO) on the cardiac anaphylactic reaction and eicosanoid release were investigated in isolated perfused sensitized guinea-pig hearts using 3-morpholinosydnonimine (SIN-1), the active metabolite of molsidomine, as NO-donor and NG-nitro-l-arginine (NNA) as an inhibitor of NO biosynthesis. Infusion of SIN-1 (final concentrations in the perfusates 0.3 or 1.0 mmol/l) elevated coronary flow under basal conditions as well as during cardiac anaphylaxis, while NNA (0.1 mmol/l) decreased basal coronary flow and aggravated the anaphylactic coronary constriction. Both drugs did not modify the characteristic biphasic profile of the coronary constriction after antigen challenge with an initial more severe phase followed by a less pronounced long-lasting flow reduction. Neither SIN-1 nor NNA affected spontaneous heart rate. However, while NNA tended to prolong the duration of antigen-induced arrhythmias, SIN-1 (1 mmol/l) had an inhibitory effect. This protection might be related to the increased coronary flow in the presence of SIN-1. SIN-1 inhibited anaphylactic release of cysteinyl-leukotrienes (LT) and 6-keto-prostaglandin (PG) F1α, but did not influence thromboxane (TX) B2 release. On the other hand, NNA (0.1 mmol/l) inhibited anaphylactic release of TXB2, but had only marginal effects on the release of cysteinyl-LT and 6-keto-PGF1α. The results suggest that exogenous and endogenous NO functionally antagonize the effects of vasoconstrictor mediators released after antigen challenge. Additional effects of high concentrations of SIN-1 and NNA on antigen-induced eicosanoid release could modulate the vascular actions of these drugs during cardiac anaphylaxis. Springer-Verlag, 1992 Cardiac anaphylaxis nationallicence Nitric oxide nationallicence 3-Morpholinosydnonimine (SIN-1) nationallicence NG-nitro- l -argi-nine (NNA) nationallicence Eicosanoids nationallicence Thelen K. Department of Pharmacology and Toxicology, Ruhr-University of Bochum, Universitätsstrasse 150, W-4630, Bochum 1, Federal Republic of Germany aut Dembinska-Kiéc A. Department of Pharmacology and Toxicology, Ruhr-University of Bochum, Universitätsstrasse 150, W-4630, Bochum 1, Federal Republic of Germany aut Pallapies D. Department of Pharmacology and Toxicology, Ruhr-University of Bochum, Universitätsstrasse 150, W-4630, Bochum 1, Federal Republic of Germany aut Simmet Th Department of Pharmacology and Toxicology, Ruhr-University of Bochum, Universitätsstrasse 150, W-4630, Bochum 1, Federal Republic of Germany aut Peskar B. Department of Pharmacology and Toxicology, Ruhr-University of Bochum, Universitätsstrasse 150, W-4630, Bochum 1, Federal Republic of Germany aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/1(1992-01-01), 93-99 0028-1298 345:1<93 1992 345 210 https://doi.org/10.1007/BF00175475 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00175475 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thelen K. Department of Pharmacology and Toxicology, Ruhr-University of Bochum, Universitätsstrasse 150, W-4630, Bochum 1, Federal Republic of Germany aut NATIONALLICENCE 700 1- Dembinska-Kiéc A. Department of Pharmacology and Toxicology, Ruhr-University of Bochum, Universitätsstrasse 150, W-4630, Bochum 1, Federal Republic of Germany aut NATIONALLICENCE 700 1- Pallapies D. Department of Pharmacology and Toxicology, Ruhr-University of Bochum, Universitätsstrasse 150, W-4630, Bochum 1, Federal Republic of Germany aut NATIONALLICENCE 700 1- Simmet Th Department of Pharmacology and Toxicology, Ruhr-University of Bochum, Universitätsstrasse 150, W-4630, Bochum 1, Federal Republic of Germany aut NATIONALLICENCE 700 1- Peskar B. Department of Pharmacology and Toxicology, Ruhr-University of Bochum, Universitätsstrasse 150, W-4630, Bochum 1, Federal Republic of Germany aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/1(1992-01-01), 93-99 0028-1298 345:1<93 1992 345 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer