caa a22 4500 469078936 CHVBK 20180323132944.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00175463 doi (NATIONALLICENCE)springer-10.1007/BF00175463 Activation of dopamine D1 receptors does not affect D2 receptor-mediated inhibition of acetylcholine release in rabbit striatum [Elektronische Daten] [Vladimir Dolezall, Rolf Jackisch, Georg Hertting, Clemens Allgaier] Summary: The possible involvement of dopamine D1 receptors in the regulation of acetylcholine release in the rabbit caudate nucleus was investigated. Caudate slices, preincubated with [3H]choline, were superfused continuously and subjected to electrical field stimulation with only a single pulse. In agreement with the view that the release of acetylcholine evoked by a single electrical pulse is not influenced by endogenous transmitters, atropine and domperidone failed to icnrease the evoked release of [3H]acetylcholine, whereas oxotremorine and quinpirole caused a concentration-dependent inhibition of transmitter release. Neither the dopamine D1 receptor antagonist SCH 23390 nor the Dt agonist SKF 38393 in a concentration range of 0.01-1 μmol/l changed the evoked [3H]acetylcholine release. The inhibitory effect of the dopamine D2 receptor agonist quinpirole was virtually abolished in the presence of 0.1 μmol/l domperidone and diminished in the presence of 1 μmol/l SCH 23390. It remained unchanged in the presence of 1 μmol/l SKF 38393. It is concluded that the inhibition of acetylcholine release by dopamine is mediated exclusively via presynaptic dopamine D2 receptors and that the antagonistic effect of SCH 23390 on the inhibition of acetylcholine release by quinpirole is due to its interaction with dopamine D2 rather than D1 receptors located on cholinergic nerve terminals. Springer-Verlag, 1992 Evoked acetylcholine release nationallicence Single pulse stimulation nationallicence Dopamine D1 receptors nationallicence Presynaptic dopamine D2 receptors nationallicence Rabbit caudate nucleus nationallicence Dolezall Vladimir Institute of Physiology, Czechoslovak Academy of Sciences, Videnskd 1083, CS-14220, Prague 4, Czechoslovakia aut Jackisch Rolf Institute of Pharmacology and Toxicology, University of Freiburg, Hermann-Herder-Strasse 5, W-7800, Federal Republic of Germany aut Hertting Georg Institute of Pharmacology and Toxicology, University of Freiburg, Hermann-Herder-Strasse 5, W-7800, Federal Republic of Germany aut Allgaier Clemens Institute of Pharmacology and Toxicology, University of Freiburg, Hermann-Herder-Strasse 5, W-7800, Federal Republic of Germany aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/1(1992-01-01), 16-20 0028-1298 345:1<16 1992 345 210 https://doi.org/10.1007/BF00175463 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00175463 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dolezall Vladimir Institute of Physiology, Czechoslovak Academy of Sciences, Videnskd 1083, CS-14220, Prague 4, Czechoslovakia aut NATIONALLICENCE 700 1- Jackisch Rolf Institute of Pharmacology and Toxicology, University of Freiburg, Hermann-Herder-Strasse 5, W-7800, Federal Republic of Germany aut NATIONALLICENCE 700 1- Hertting Georg Institute of Pharmacology and Toxicology, University of Freiburg, Hermann-Herder-Strasse 5, W-7800, Federal Republic of Germany aut NATIONALLICENCE 700 1- Allgaier Clemens Institute of Pharmacology and Toxicology, University of Freiburg, Hermann-Herder-Strasse 5, W-7800, Federal Republic of Germany aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/1(1992-01-01), 16-20 0028-1298 345:1<16 1992 345 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer