caa a22 4500 469078960 CHVBK 20180323132944.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00175467 doi (NATIONALLICENCE)springer-10.1007/BF00175467 Prejunctional opioid μ-receptors and adenosine A1-receptors on the sympathetic nerve endings of the rat tail artery interact with the α2-adrenoceptors [Elektronische Daten] [B. Bucher, C. Corriu, J. Stoclet] Summary: Experiments were designed to study the interaction between prejunctional α2-adrenoceptors and both adenosine and opioid receptors at the postganglionic sympathetic nerve endings innervating the tail artery of the rat. Segments of this vessel were preincubated with [3H]-noradrenaline and then perfused/superfused with [3H]-noradrenaline-free medium. Their perivascular nerves were field stimulated with standard stimulation parameters: 24 pulses at 0.4 Hz, 0.3 ms, 200 mA. In some experiments, the stimulation parameters were adjusted in order to obtain similar reference release values despite the presence of a first release-modulating drug. The adenosine agonist 5′-N-ethylcarboxamidoadenosine (NECA; 0.3-10 μmol/l) and [D-Ala2,MePhe4,Glyol5]enkephalin (DAGO; 0.3-10 μmol/l) depressed the stimulation-evoked overflow of tritium in a concentration dependent manner. The release-inhibiting effect of both NECA and DAGO was enhanced in the presence of the α2-adrenoceptor antagonist rauwolscine (3 μmol/l) while it was attenuated in the presence of the α2-adrenoceptor agonist 5-bromo-6-[2-imidazolin-2yl-aminol-quinoxaline (UK-14,304; 0.1 μmol/l). These changes occurred both at standard and adjusted stimulation parameters. These results demonstrate that the prejunctional adenosine A1- and opioid μ-receptors interact with the prejunctional α2-adrenoceptors. The level at which these interactions take place (receptors themselves or transduction mechanisms) as well as the physiological significance of the phenomenon remain to be determined. Springer-Verlag, 1992 Rat tail artery nationallicence Noradrenaline release nationallicence Prejunctional receptor interactions nationallicence α2-Adrenoceptors nationallicence Adenosine receptors nationallicence Opioid receptors nationallicence Bucher B. Université Louis Pasteur, Strasbourg, Laboratoire de Pharmacologie Cellulaire et Moléculaire, C.N.R.S. URA 600, B.P. 24, 74, route du Rhin, F-67401, Illkirch Cedex, France aut Corriu C. Université Louis Pasteur, Strasbourg, Laboratoire de Pharmacologie Cellulaire et Moléculaire, C.N.R.S. URA 600, B.P. 24, 74, route du Rhin, F-67401, Illkirch Cedex, France aut Stoclet J. Université Louis Pasteur, Strasbourg, Laboratoire de Pharmacologie Cellulaire et Moléculaire, C.N.R.S. URA 600, B.P. 24, 74, route du Rhin, F-67401, Illkirch Cedex, France aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/1(1992-01-01), 37-43 0028-1298 345:1<37 1992 345 210 https://doi.org/10.1007/BF00175467 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00175467 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bucher B. Université Louis Pasteur, Strasbourg, Laboratoire de Pharmacologie Cellulaire et Moléculaire, C.N.R.S. URA 600, B.P. 24, 74, route du Rhin, F-67401, Illkirch Cedex, France aut NATIONALLICENCE 700 1- Corriu C. Université Louis Pasteur, Strasbourg, Laboratoire de Pharmacologie Cellulaire et Moléculaire, C.N.R.S. URA 600, B.P. 24, 74, route du Rhin, F-67401, Illkirch Cedex, France aut NATIONALLICENCE 700 1- Stoclet J. Université Louis Pasteur, Strasbourg, Laboratoire de Pharmacologie Cellulaire et Moléculaire, C.N.R.S. URA 600, B.P. 24, 74, route du Rhin, F-67401, Illkirch Cedex, France aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/1(1992-01-01), 37-43 0028-1298 345:1<37 1992 345 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer