caa a22 4500 469079169 CHVBK 20180323132944.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00164576 doi (NATIONALLICENCE)springer-10.1007/BF00164576 Preferential location of N-methyl-D-aspartate (NMDA) receptors on postsynaptic membranes and on non-noradrenergic nerve terminals of the rat brain cortex [Elektronische Daten] [K. Fink, M. Blohm, G. Molderings, H. Bönisch, M. Göthert] Summary: The effect of DSP4-induced destruction of noradrenergic neurones on 3H-3-(2-carboxypiperazine-4-yl)propyl-1-phosphonic acid (3H-CPP) binding to N-methyl-D-aspartate (NMDA) receptors and on 3H-desipramine (3H-DMI) binding to the neuronal noradrenaline carrier was investigated in rat brain cortex buffy coat membranes. 3H-DMI bound with high affinity to a single site at the neuronal noradrenaline carrier (KD = 5.26 ± 1.67 nmol/l) whereas the binding of 3H-CPP to the NMDA receptor was of intermediate affinity (KD = 274 ± 45 nmol/l). Fourteen days after a single-dose treatment with DSP4 (1) the Bmax value for 3H-DMI binding was reduced by 74%, (2) the Bmax value for 3H-CPP binding only tended to be decreased (by 24%; not statistically significant), (3) the endogenous noradrenaline content was reduced by 70% compared to untreated controls and, (4) the absolute amount of the NMDA-evoked 3H-noradrenaline overflow but not the fractional release was reduced by 55%. It is concluded that in the rat cerebral cortex presynaptic NMDA-receptors on noradrenergic nerve endings, which have previously been detected in release experiments with NMDA on cortical synaptosomes preincubated with 3H-noradrenaline, cannot be identified in radioligand binding experiments. Obviously, the cerebral cortical NMDA receptors are predominantly located on postsynaptic neuronal membranes and potentially on non-noradrenergic nerve terminals as well. Springer-Verlag, 1992 NMDA receptor nationallicence 3H-CPP binding nationallicence 3H-Desipramine binding nationallicence DSP4 nationallicence Rat brain cortex nationallicence Fink K. Institut für Pharmakologie und Toxikologie der Rheinischen Friedrich-Wilhelms-Universität Bonn, Reuterstrasse 2b, W-5300, Bonn 1, Federal Republic of Germany aut Blohm M. Institut für Pharmakologie und Toxikologie der Rheinischen Friedrich-Wilhelms-Universität Bonn, Reuterstrasse 2b, W-5300, Bonn 1, Federal Republic of Germany aut Molderings G. Institut für Pharmakologie und Toxikologie der Rheinischen Friedrich-Wilhelms-Universität Bonn, Reuterstrasse 2b, W-5300, Bonn 1, Federal Republic of Germany aut Bönisch H. Institut für Pharmakologie und Toxikologie der Rheinischen Friedrich-Wilhelms-Universität Bonn, Reuterstrasse 2b, W-5300, Bonn 1, Federal Republic of Germany aut Göthert M. Institut für Pharmakologie und Toxikologie der Rheinischen Friedrich-Wilhelms-Universität Bonn, Reuterstrasse 2b, W-5300, Bonn 1, Federal Republic of Germany aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/6(1992-06-01), 633-638 0028-1298 345:6<633 1992 345 210 https://doi.org/10.1007/BF00164576 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164576 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fink K. Institut für Pharmakologie und Toxikologie der Rheinischen Friedrich-Wilhelms-Universität Bonn, Reuterstrasse 2b, W-5300, Bonn 1, Federal Republic of Germany aut NATIONALLICENCE 700 1- Blohm M. Institut für Pharmakologie und Toxikologie der Rheinischen Friedrich-Wilhelms-Universität Bonn, Reuterstrasse 2b, W-5300, Bonn 1, Federal Republic of Germany aut NATIONALLICENCE 700 1- Molderings G. Institut für Pharmakologie und Toxikologie der Rheinischen Friedrich-Wilhelms-Universität Bonn, Reuterstrasse 2b, W-5300, Bonn 1, Federal Republic of Germany aut NATIONALLICENCE 700 1- Bönisch H. Institut für Pharmakologie und Toxikologie der Rheinischen Friedrich-Wilhelms-Universität Bonn, Reuterstrasse 2b, W-5300, Bonn 1, Federal Republic of Germany aut NATIONALLICENCE 700 1- Göthert M. Institut für Pharmakologie und Toxikologie der Rheinischen Friedrich-Wilhelms-Universität Bonn, Reuterstrasse 2b, W-5300, Bonn 1, Federal Republic of Germany aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/6(1992-06-01), 633-638 0028-1298 345:6<633 1992 345 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer