caa a22 4500 469079193 CHVBK 20180323132944.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00168687 doi (NATIONALLICENCE)springer-10.1007/BF00168687 Effect of tianeptine on the central cholinergic system: involvement of serotonin [Elektronische Daten] [Rosalia Bertorelli, Daniela Amoroso, Prisca Girotti, Silvana Consolo] Summary: The effect of tianeptine on in vivo acetylcholine (ACh) release from brain hemispheric regions of freely moving rats was investigated using the microdialysis technique coupled with a sensitive radioenzymatic method. Tianeptine, at the dose of 30 mg/kgi.p., reduced ACh release from dorsal hippocampi by 40% in 40 min, and induced a 30% decrease of ACh output from frontal cortices while at the doses of 10 and 20 mg/kg it had no effect. In striata the drug did not significantly affect ACh release although it showed a tendency to increase it. The ACh content in the three areas considered was not affected by tianeptine at above doses. The drug did not alter choline-o-acetyltransferase and acetylcholinesterase activities suggesting that it did not influence the cholinergic system through direct action on the ACh metabolism; furthermore, it did not influence the sodium-dependent high-affinity uptake of choline in striatum, cortex and hippocampus. Impairment of serotonergic (5-HT) neurotransmission by chemical lesion of the median raphe nucleus or by metergoline, a blocker of 5-HT receptors, antagonized the cholinergic effect of tianeptine. The involvement of the serotonergic system is specific because lesions of the noradrenergic dorsal bundle failed to prevent the inhibitory action of tianeptine. The present data suggest that 5-HT may mediate the effect of tianeptine on the cholinergic system in dorsal hippocampi. Springer-Verlag, 1992 Acetylcholine release in vivo nationallicence Serotonin nationallicence Microdialysis nationallicence Rat brain hemispheric regions nationallicence Bertorelli Rosalia Istituto di Ricerche Farmacologiche ‘Mario Negri', Via Eritrea 62, I-20157, Milan, Italy aut Amoroso Daniela Istituto di Ricerche Farmacologiche ‘Mario Negri', Via Eritrea 62, I-20157, Milan, Italy aut Girotti Prisca Istituto di Ricerche Farmacologiche ‘Mario Negri', Via Eritrea 62, I-20157, Milan, Italy aut Consolo Silvana Istituto di Ricerche Farmacologiche ‘Mario Negri', Via Eritrea 62, I-20157, Milan, Italy aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/3(1992-03-01), 276-281 0028-1298 345:3<276 1992 345 210 https://doi.org/10.1007/BF00168687 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00168687 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bertorelli Rosalia Istituto di Ricerche Farmacologiche ‘Mario Negri', Via Eritrea 62, I-20157, Milan, Italy aut NATIONALLICENCE 700 1- Amoroso Daniela Istituto di Ricerche Farmacologiche ‘Mario Negri', Via Eritrea 62, I-20157, Milan, Italy aut NATIONALLICENCE 700 1- Girotti Prisca Istituto di Ricerche Farmacologiche ‘Mario Negri', Via Eritrea 62, I-20157, Milan, Italy aut NATIONALLICENCE 700 1- Consolo Silvana Istituto di Ricerche Farmacologiche ‘Mario Negri', Via Eritrea 62, I-20157, Milan, Italy aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/3(1992-03-01), 276-281 0028-1298 345:3<276 1992 345 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer