caa a22 4500 469079258 CHVBK 20180323132945.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00168698 doi (NATIONALLICENCE)springer-10.1007/BF00168698 Difference between aortic and renal vascular reactivity in cyclosporin A treated rats and the effect of cicletanine [Elektronische Daten] [E. Mikkelsen, S. Poulsen, N. Nyborg, N. Korsgaard, J. Sehested] Summary: 4 Groups of 2 month-old male Wistar rats were treated with a) cyclosporin A (CyA) 30 mg/kg/day alone, b) CyA plus cicletanine (Cic) 60 mg/kg/day, c) vehicle (vegetable oil) 1 ml/100 g rat/day and d) no treatment for 8 weeks. The reactivity of isolated papillary muscle to isoprenaline and Ca 2+ was not altered in any of the treated groups. Endothelium-dependent relaxation induced by acetylcholine was inhibited in aorta ring segments from CyA treated rats as compared to that of control and CyA+Cic-treated rats. The relaxation induced by acetylcholine in rat aortas was similar in all groups in the presence of 10 μM indomethacin. Noradrenaline sensitivity of aortic segments was not affected by any treatments applied. The Ca2+-concentration response curves of aorta segments from CyA-treated and CyA+Cic-treated rats were shifted to the right as compared to control rats. In interlobar renal arteries the endothelium-dependent relaxation induced by acetylcholine was not affected by any form of treatment. In renal arteries 10 μM indomethacin increased the maximal relaxation induced by acetylcholine about 50%. In these vessels noradrenaline sensitivity in CyA and CyA+Cic treated rats was higher than in controls. Cocaine, 3 μM, shifted the noradrenaline concentration response curve to the left about 0.4 log units in all renal vessel groups, thus renal vascular smooth muscle sensitivity to noradrenaline was significantly greater in vessels from rats receiving CyA than in vessels from control rats. Administration of CyA induced only slight renal morphological changes. Cic was without effect on CyA induced morphological abnormalities. The results indicate that Cic is able to reverse the inhibitory effect of CyA on endothelium-dependent relaxations in aorta but does not affect the increased noradrenaline sensitivity of renal arteries associated with CyA treatment and was without effect on CyA induced changes in renal morphology. Treatment with CyA alone or in combination with Cic had no effect on the contractile force of the papillary muscle, indicating that CyA only affects function of vascular but not cardiac tissue. Springer-Verlag, 1992 Cyclosporin A nationallicence Cicletanine nationallicence Vascular smooth muscle nationallicence Cardiac muscle nationallicence Wistar rats nationallicence Mikkelsen E. Department of Pharmacology, Aarhus University, DK-8000, Aarhus C, Denmark aut Poulsen S. Department of Pharmacology, Aarhus University, DK-8000, Aarhus C, Denmark aut Nyborg N. Department of Pharmacology, Aarhus University, DK-8000, Aarhus C, Denmark aut Korsgaard N. Department of Pathology, Aarhus University, DK-8000, Aarhus C, Denmark aut Sehested J. Deutsches Herzzentrum Berlin, W-1000, Berlin, Federal Republic of Germany aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/3(1992-03-01), 356-361 0028-1298 345:3<356 1992 345 210 https://doi.org/10.1007/BF00168698 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00168698 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mikkelsen E. Department of Pharmacology, Aarhus University, DK-8000, Aarhus C, Denmark aut NATIONALLICENCE 700 1- Poulsen S. Department of Pharmacology, Aarhus University, DK-8000, Aarhus C, Denmark aut NATIONALLICENCE 700 1- Nyborg N. Department of Pharmacology, Aarhus University, DK-8000, Aarhus C, Denmark aut NATIONALLICENCE 700 1- Korsgaard N. Department of Pathology, Aarhus University, DK-8000, Aarhus C, Denmark aut NATIONALLICENCE 700 1- Sehested J. Deutsches Herzzentrum Berlin, W-1000, Berlin, Federal Republic of Germany aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/3(1992-03-01), 356-361 0028-1298 345:3<356 1992 345 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer