caa a22 4500 469079266 CHVBK 20180323132945.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00168690 doi (NATIONALLICENCE)springer-10.1007/BF00168690 Carbachol potentiates isoprenaline-induced mucin secretion by rat submandibular gland [Elektronische Daten] [Samuel Taylor, Le Nguyen, Christine Halket] Summary: We studied the effects of carbachol on isoprenaline-induced secretion of 3[H]glucosamine-labeled high molecular weight protein from in vitro fragments of rat submandibular gland. The concentration-response curve for isoprenaline was determined alone and in the presence of one of two concentrations of carbachol. Isobolic interaction indices were calculated from the resulting curves. The role of extracellular calcium on this interaction was assessed by examining the effect of increasing concentrations of [Ca2+]e on secretion induced by a fixed concentration of isoprenaline alone and in the presence of carbachol. Carbachol alone caused a small, but statistically significant, protein secretion. Carbachol markedly shifted the isoprenaline concentration-response curve to the left in a dose-dependent manner. In the absence of extracellular calcium, carbachol did not increase isoprenaline-induced secretion, but secretion increased as the [Ca2+]e was increased. The carbachol-induced increase in secretion reached a maximal level at approximately 2.0 mM [Ca2+]e. We conclude that carbachol's increase of isoprenaline-induced secretion of 3[H]glucosamine-labeled high molecular weight protein is not additive, but is true potentiation. This potentiation requires extracellular calcium, and may be maximal at physiological [Ca2+]e. Springer-Verlag, 1992 β-Adrenergic nationallicence Submandibular gland nationallicence Mucin nationallicence Drug interaction nationallicence Taylor Samuel Department of Pharmacology, Baylor College of Dentistry, 3302 Gaston Avenue, 75246, Dallas, TX, USA aut Nguyen Le Department of Pharmacology, Baylor College of Dentistry, 3302 Gaston Avenue, 75246, Dallas, TX, USA aut Halket Christine Department of Pharmacology, Baylor College of Dentistry, 3302 Gaston Avenue, 75246, Dallas, TX, USA aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/3(1992-03-01), 296-299 0028-1298 345:3<296 1992 345 210 https://doi.org/10.1007/BF00168690 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00168690 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Taylor Samuel Department of Pharmacology, Baylor College of Dentistry, 3302 Gaston Avenue, 75246, Dallas, TX, USA aut NATIONALLICENCE 700 1- Nguyen Le Department of Pharmacology, Baylor College of Dentistry, 3302 Gaston Avenue, 75246, Dallas, TX, USA aut NATIONALLICENCE 700 1- Halket Christine Department of Pharmacology, Baylor College of Dentistry, 3302 Gaston Avenue, 75246, Dallas, TX, USA aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/3(1992-03-01), 296-299 0028-1298 345:3<296 1992 345 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer