caa a22 4500 46907972X CHVBK 20180323132946.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00168956 doi (NATIONALLICENCE)springer-10.1007/BF00168956 Pertussis toxin abolishes the inhibition of Ca2+ currents and of noradrenaline release via α2-adrenoceptors in chick sympathetic neurons [Elektronische Daten] [S. Boehm, S. Huck, H. Drobny, E.A. Singer] Summary: Effects of α2-adrenoceptor agonists on whole-cell Ca2+ currents and 3H-noradrenaline release were investigated by applying the patch-clamp technique and electrical field stimulation to cultured embryonic chick sympathetic neurons. A 24-h exposure of the sympathetic neurons to pertussis toxin (100 ng/ml) abolished both the α2-adrenoceptor-mediated inhibition of Ca 2+ currents and the modulation of noradrenaline release caused by noradrenaline (1 μmol/l; in the presence of 10 μmol/l cocaine) or the α2-adrenoceptor agonists 5-bromo-6-(2imidazolin-2-ylamino)quinoxaline (UK 14,304, 10 μmol/ l) and clonidine (10 μmol/l). These results suggest that the α2-autoreceptor-mediated inhibition of noradrenaline release from chick sympathetic neurons operates through the modulation of Ca2+ channels via pertussistoxin-sensitive GTP-binding-proteins. Springer-Verlag, 1992 Chick sympathetic neurons nationallicence α2-Adrenoceptors nationallicence Pertussis toxin nationallicence Ca2+ currents nationallicence H-noradrenaline release nationallicence Boehm S. Institute of Neuropharmacology, University of Vienna, Waehringerstrasse 13a, A-1090, Vienna, Austria aut Huck S. Institute of Neuropharmacology, University of Vienna, Waehringerstrasse 13a, A-1090, Vienna, Austria aut Drobny H. Institute of Pharmacology, University of Vienna, Waehringerstrasse 13a, A-1090, Vienna, Austria aut Singer E.A. Institute of Pharmacology, University of Vienna, Waehringerstrasse 13a, A-1090, Vienna, Austria aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/5(1992-05-01), 606-609 0028-1298 345:5<606 1992 345 210 https://doi.org/10.1007/BF00168956 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00168956 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boehm S. Institute of Neuropharmacology, University of Vienna, Waehringerstrasse 13a, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Huck S. Institute of Neuropharmacology, University of Vienna, Waehringerstrasse 13a, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Drobny H. Institute of Pharmacology, University of Vienna, Waehringerstrasse 13a, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Singer E.A. Institute of Pharmacology, University of Vienna, Waehringerstrasse 13a, A-1090, Vienna, Austria aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/5(1992-05-01), 606-609 0028-1298 345:5<606 1992 345 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer