caa a22 4500 469079835 CHVBK 20180323132947.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00165745 doi (NATIONALLICENCE)springer-10.1007/BF00165745 Muscarinic cholinergic receptors in the human right coronary artery: a receptor binding and autoradiographic study [Elektronische Daten] [Francesco Amenta, Manuela De Michele, Pada Strocchi, Fabio Ferrante, Piero Gallo] Summary: We used a combination of radioreceptor binding and autoradiographic techniques to study the pharmacological characteristics and anatomical localization of [3H]-quinuclidinyl benzilate (QNB) binding sites in the human right coronary artery. The ligand was bound to sections of the human right coronary artery in a manner consistent with the labelling of muscarinic receptors. The addition of pirenzepine or of carbachol to the incubation medium to generate displacement curves was indicative of the presence of M1 and M2 receptors in the right coronary artery. Autoradiography showed the localization of M1 sites primarily in the medial layer of the right coronary artery. M2 sites were located primarily in the adventitia. No [3H]-QNB binding sites were observed in the endothelium. A possible role of muscarinic receptors in the pathogenesis of coronary vasospasm is discussed. Springer-Verlag, 1992 Muscarinic receptors nationallicence Coronary artery nationallicence Receptor binding nationallicence Autoradiography nationallicence Human nationallicence Amenta Francesco Dipartimento di Sanità Pubblica e Biologia Cellulare, Università "Tor Vergata”, Rome, Italy aut De Michele Manuela Dipartimento di Scienze Cardiovascolari e Respiratorie, Università "La Sapienza”, Rome, Italy aut Strocchi Pada Dipartimento di Sanità Pubblica e Biologia Cellulare, Università "Tor Vergata”, Rome, Italy aut Ferrante Fabio Dipartimento di Scienze Cardiovascolari e Respiratorie, Università "La Sapienza”, Rome, Italy aut Gallo Piero Dipartimento di Biopatologia Umana, Università "La Sapienza”, Rome, Italy aut Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/2(1992-02-01), 251-254 0028-1298 345:2<251 1992 345 210 https://doi.org/10.1007/BF00165745 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00165745 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Amenta Francesco Dipartimento di Sanità Pubblica e Biologia Cellulare, Università "Tor Vergata”, Rome, Italy aut NATIONALLICENCE 700 1- De Michele Manuela Dipartimento di Scienze Cardiovascolari e Respiratorie, Università "La Sapienza”, Rome, Italy aut NATIONALLICENCE 700 1- Strocchi Pada Dipartimento di Sanità Pubblica e Biologia Cellulare, Università "Tor Vergata”, Rome, Italy aut NATIONALLICENCE 700 1- Ferrante Fabio Dipartimento di Scienze Cardiovascolari e Respiratorie, Università "La Sapienza”, Rome, Italy aut NATIONALLICENCE 700 1- Gallo Piero Dipartimento di Biopatologia Umana, Università "La Sapienza”, Rome, Italy aut NATIONALLICENCE 773 0- Naunyn-Schmiedeberg's Archives of Pharmacology Springer-Verlag 345/2(1992-02-01), 251-254 0028-1298 345:2<251 1992 345 210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer