caa a22 4500 469080116 CHVBK 20180323132947.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00569329 doi (NATIONALLICENCE)springer-10.1007/BF00569329 Heterogeneity of the hemoglobin of the Ohrid trout ( Salmo L. typicus ) [Elektronische Daten] [Biljana Cepreganova, Jerry Wilson, Brooke Webber, Biljana Kjovkareska, Georgi Efremov, Titus Huisman] We have analyzed the hemoglobins of five individual trout from the Ohrid Lake (Salmo L. typicus) by electrophoretic methods, by reversed-phase high-performance liquid chromatography, and by limited structural analyses. The two major classes of hemoglobin are type I (35% of total) and type IV (65%). Type IV is the major oxygen-transporting hemoglobin; it consists of three types of β chain (in about equal quantities) and three types of α chain (one major and two minor types). Several structural differences have been observed between these three β(IV) chains and between the three α(IV) chains, suggesting a complex genetic system governing the synthesis of these proteins. Moreover, a few amino acid substitutions occur at positions involved in contacts between chains, which suggests that differences in oxygen affinity may exist between these various type IV hemoglobins. Type I hemoglobin is less complex because it contains one type of β chain and two α chains; the latter two differ in numerous positions, suggesting duplications of the α(I)-globin gene. The α and β chains of type I hemoglobin differ considerably from the α and β chains of type IV hemoglobin, indicating the existence of α(I)- and β(I)-globin genes separate from the α(IV)- and β(IV)- globin genes. Plenum Publishing Corporation, 1992 hemoglobin nationallicence type I nationallicence type IV nationallicence trout ( Salmo L. typicus ) nationallicence Cepreganova Biljana Laboratory of Protein Chemistry, Department of Biochemistry and Molecular Biology, Medical College of Georgia, 30912-2100, Augusta, Georgia aut Wilson Jerry Laboratory of Protein Chemistry, Department of Biochemistry and Molecular Biology, Medical College of Georgia, 30912-2100, Augusta, Georgia aut Webber Brooke Laboratory of Protein Chemistry, Department of Biochemistry and Molecular Biology, Medical College of Georgia, 30912-2100, Augusta, Georgia aut Kjovkareska Biljana Macedonian Academy of Sciences and Arts, Research Center for New Technologies, 9100, Skopje, Macedonia aut Efremov Georgi Macedonian Academy of Sciences and Arts, Research Center for New Technologies, 9100, Skopje, Macedonia aut Huisman Titus Laboratory of Protein Chemistry, Department of Biochemistry and Molecular Biology, Medical College of Georgia, 30912-2100, Augusta, Georgia aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 30/7-8(1992-08-01), 385-399 0006-2928 30:7-8<385 1992 30 10528 https://doi.org/10.1007/BF00569329 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00569329 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cepreganova Biljana Laboratory of Protein Chemistry, Department of Biochemistry and Molecular Biology, Medical College of Georgia, 30912-2100, Augusta, Georgia aut NATIONALLICENCE 700 1- Wilson Jerry Laboratory of Protein Chemistry, Department of Biochemistry and Molecular Biology, Medical College of Georgia, 30912-2100, Augusta, Georgia aut NATIONALLICENCE 700 1- Webber Brooke Laboratory of Protein Chemistry, Department of Biochemistry and Molecular Biology, Medical College of Georgia, 30912-2100, Augusta, Georgia aut NATIONALLICENCE 700 1- Kjovkareska Biljana Macedonian Academy of Sciences and Arts, Research Center for New Technologies, 9100, Skopje, Macedonia aut NATIONALLICENCE 700 1- Efremov Georgi Macedonian Academy of Sciences and Arts, Research Center for New Technologies, 9100, Skopje, Macedonia aut NATIONALLICENCE 700 1- Huisman Titus Laboratory of Protein Chemistry, Department of Biochemistry and Molecular Biology, Medical College of Georgia, 30912-2100, Augusta, Georgia aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 30/7-8(1992-08-01), 385-399 0006-2928 30:7-8<385 1992 30 10528 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer