caa a22 4500 469080167 CHVBK 20180323132948.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00569327 doi (NATIONALLICENCE)springer-10.1007/BF00569327 Restriction fragment length variants in the marsupial Sminthopsis crassicaudata [Elektronische Daten] [Rory Hope, Henry Bennett, Clive Chesson, Steven Cooper] A fully pedigreed colony of the dasyurid marsupialSminthopsis crassicaudata has provided material for establishing two panels of DNA samples: a broad-based test panel and a two-generation family panel. These have been used to search for genetic markers in the form of restriction fragment length variants. The molecular probes—pSG-2H, a region of theS. crassicaudata embryonic β-globin gene; pB8.BS, a region of the human ubiquitin gene, and p3-21a1:1, a region of the processed pseudogene of phosphoglycerate kinase-1 of the macropodid marsupialMacropus robustus—were hybridized to Southern blots ofEcoR1-digested DNA from the panels. Analysis of these blots when probed with pSG-2H provided evidence of two alleles segregating at a singleEcoR1 site. Analysis of the same blots when probed with pB8.BS suggested allelic variation at two closely linkedEcoR1 sites. Probing the blots with p3-21a1:1 produced a complex pattern of bands resembling DNA fingerprints. The presence of a 12.3-kb band was found to conform to a simple autosomal dominant mode of inheritance. Analysis of the family data, for each probe, revealed no significant departure from Mendelian inheritance. This work has provided additional genetic markers that will enhance the use ofS. crassicaudata as a model marsupial species and has demonstrated that a high level of genetic variability has been maintained in the marsupial colony. Plenum Publishing Corporation, 1992 Sminthopsis crassicaudata nationallicence marsupial nationallicence pedigreed colony nationallicence DNA nationallicence restriction fragment length variants nationallicence Mendelian inheritance nationallicence mammalian evolution nationallicence Hope Rory Department of Genetics, University of Adelaide, Box 498 GPO, 5001, Adelaide, South Australia, Australia aut Bennett Henry Department of Genetics, University of Adelaide, Box 498 GPO, 5001, Adelaide, South Australia, Australia aut Chesson Clive Department of Genetics, University of Adelaide, Box 498 GPO, 5001, Adelaide, South Australia, Australia aut Cooper Steven Department of Genetics, University of Adelaide, Box 498 GPO, 5001, Adelaide, South Australia, Australia aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 30/7-8(1992-08-01), 361-370 0006-2928 30:7-8<361 1992 30 10528 https://doi.org/10.1007/BF00569327 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00569327 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hope Rory Department of Genetics, University of Adelaide, Box 498 GPO, 5001, Adelaide, South Australia, Australia aut NATIONALLICENCE 700 1- Bennett Henry Department of Genetics, University of Adelaide, Box 498 GPO, 5001, Adelaide, South Australia, Australia aut NATIONALLICENCE 700 1- Chesson Clive Department of Genetics, University of Adelaide, Box 498 GPO, 5001, Adelaide, South Australia, Australia aut NATIONALLICENCE 700 1- Cooper Steven Department of Genetics, University of Adelaide, Box 498 GPO, 5001, Adelaide, South Australia, Australia aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 30/7-8(1992-08-01), 361-370 0006-2928 30:7-8<361 1992 30 10528 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer