caa a22 4500 46908040X CHVBK 20180323132948.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00554430 doi (NATIONALLICENCE)springer-10.1007/BF00554430 Restriction fragment analysis of the secalin loci of rye [Elektronische Daten] [Gillian Hull, Paolo Sabelli, Peter Shewry] Analyses of wheat/rye addition lines by Southern blotting confirmed the presence of sequences related to theSec 1, Sec 2, andSec 3 loci on chromosomes 1R and 2R. Comparison of the 1R and 2R addition lines allowed the identification of γ-secalin genes atSec 1 andSec 2, respectively, while ω-secalin and γ-secalin genes atSec 1 were discriminated by comparative hybridization with three probes: ω-secalin, total γ-secalin, and 3′ γ-secalin. The high molecular weight (HMW) secalin genes atSec 3 were identified using a homologous HMW subunit probe from wheat. Gene copy numbers were estimated as about 40-60 for ω-secalins, 5-10 for γ-secalins, and 2 for HMW secalins. Comparison of individual plants of cv. Gazelle showed a high degree of polymorphism, particularly for sequences related to ω-secalins and HMW secalins. Plenum Publishing Corporation, 1992 restriction fragment length polymorphisms (RFLPs) nationallicence Southern blotting nationallicence polymorphism nationallicence addition lines nationallicence DNA probes nationallicence Hull Gillian Rothamsted Experimental Station, AFRC Institute of Arable Crops Research, AL5 2JQ, Harpenden, Herts, UK aut Sabelli Paolo Department of Agricultural Sciences, University of Bristol, AFRC Institute of Arable Crops Research, Long Ashton Research Station, BS18 9AF, Bristol, UK aut Shewry Peter Department of Agricultural Sciences, University of Bristol, AFRC Institute of Arable Crops Research, Long Ashton Research Station, BS18 9AF, Bristol, UK aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 30/1-2(1992-02-01), 85-97 0006-2928 30:1-2<85 1992 30 10528 https://doi.org/10.1007/BF00554430 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00554430 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hull Gillian Rothamsted Experimental Station, AFRC Institute of Arable Crops Research, AL5 2JQ, Harpenden, Herts, UK aut NATIONALLICENCE 700 1- Sabelli Paolo Department of Agricultural Sciences, University of Bristol, AFRC Institute of Arable Crops Research, Long Ashton Research Station, BS18 9AF, Bristol, UK aut NATIONALLICENCE 700 1- Shewry Peter Department of Agricultural Sciences, University of Bristol, AFRC Institute of Arable Crops Research, Long Ashton Research Station, BS18 9AF, Bristol, UK aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 30/1-2(1992-02-01), 85-97 0006-2928 30:1-2<85 1992 30 10528 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer