caa a22 4500 469080442 CHVBK 20180323132948.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00554424 doi (NATIONALLICENCE)springer-10.1007/BF00554424 Identification of two distinct amplifications of the esterase B locus in Culex pipiens (L.) mosquitoes from mediterranean countries [Elektronische Daten] [Marylène Poirié, Michel Raymond, Nicole Pasteur] Two new highly active esterases were detected by starch electrophoretic studies inCulex pipiens mosquitoes from the area of Montpellier (France) and from Cyprus. We demonstrate here that both the French and the Cyprus esterases B are overproduced due to amplification of the coding gene. The production of the esterase B is approximately 50- and 500-fold higher in mosquitoes from France and Cyprus, respectively, than in susceptible insects, whereas the number of gene copies is about 25 and 250. Differences of about 7- and 95-fold were also found in the degree of chlorpyrifos resistance. RFLP comparison of the amplified region containing the esterase B gene revealed large differences between French and Cyprus mosquitoes. It thus appears that two distinct haplotypes with an esterase B gene coding an enzyme with identical electrophoretic mobility have been amplified. We therefore named the haplotypes in mosquitoes from France and Cyprus B4 and B5, respectively. The estimated genetic distance between these two haplotypes is not smaller than those observed in all pair comparisons of other known esterase B haplotypes. These results are discussed in the context of amplification phenomena. Plenum Publishing Corporation, 1992 mosquito nationallicence esterase B locus nationallicence amplification nationallicence Poirié Marylène Laboratoire de Génétique et Environnement, Case courrier 64, Institut des Sciences de l'Evolution (CNRS, URA 327), Université Montpellier II, 34095, Montpellier 05, France aut Raymond Michel Laboratoire de Génétique et Environnement, Case courrier 64, Institut des Sciences de l'Evolution (CNRS, URA 327), Université Montpellier II, 34095, Montpellier 05, France aut Pasteur Nicole Laboratoire de Génétique et Environnement, Case courrier 64, Institut des Sciences de l'Evolution (CNRS, URA 327), Université Montpellier II, 34095, Montpellier 05, France aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 30/1-2(1992-02-01), 13-26 0006-2928 30:1-2<13 1992 30 10528 https://doi.org/10.1007/BF00554424 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00554424 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Poirié Marylène Laboratoire de Génétique et Environnement, Case courrier 64, Institut des Sciences de l'Evolution (CNRS, URA 327), Université Montpellier II, 34095, Montpellier 05, France aut NATIONALLICENCE 700 1- Raymond Michel Laboratoire de Génétique et Environnement, Case courrier 64, Institut des Sciences de l'Evolution (CNRS, URA 327), Université Montpellier II, 34095, Montpellier 05, France aut NATIONALLICENCE 700 1- Pasteur Nicole Laboratoire de Génétique et Environnement, Case courrier 64, Institut des Sciences de l'Evolution (CNRS, URA 327), Université Montpellier II, 34095, Montpellier 05, France aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 30/1-2(1992-02-01), 13-26 0006-2928 30:1-2<13 1992 30 10528 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer