caa a22 4500 469080469 CHVBK 20180323132948.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00554431 doi (NATIONALLICENCE)springer-10.1007/BF00554431 Duplication of the structural gene for glucosephosphate isomerase and phosphogluconate dehydrogenase in Scabiosa columbaria and their phylogenetic implications in the dipsacaceae [Elektronische Daten] [R. Van Treuren, R. Bijlsma] Zymograms of glucosephosphate isomerase (GPI) and phosphogluconate dehydrogenase (PGD) revealed three isozymes for each enzyme in the plant speciesScabiosa columbaria. Intergenic heterodimers are formed between the polypeptides coded byGpi-1 andGpi-2 and between those coded byPgd-1 andPgd-2, indicating that a GPI and a PGD locus have been duplicated in the past. The ancestral genes assort independently with their duplicated gene, suggesting that the duplications have originated from a process of translocation. Linkage was found only betweenGpi-1 andPgd-2 and betweenGpi-2 andPgd-1, suggesting that the duplicated loci were located on the same translocated chromosomal segment. Both duplications are present in all other examined species ofScabiosa and inCephalaria andKnautia, two other genera of the Dipsacaceae. The generaSuccisa andDipsacus, also belonging to the Dipsacaceae, do not showGpi-1 activity, makingGpi-2 andPgd-1 the most likely ancestral genes. InSuccisa, the isozymes ofGpi-1 andGpi-2 either overlap orGpi-1 has been silenced. The combined results suggest that a chromosomal segment containingGpi-2 andPgd-1 has been translocated before the divergence ofScabiosa, Cephalaria, Knautia, andSuccisa. Plenum Publishing Corporation, 1992 gene duplication nationallicence glucosephosphate isomerase nationallicence phosphogluconate dehydrogenase nationallicence gene linkage nationallicence Scabiosa columbaria nationallicence electrophoresis nationallicence phylogenetics nationallicence Van Treuren R. Department of Genetics, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands aut Bijlsma R. Department of Genetics, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 30/1-2(1992-02-01), 99-109 0006-2928 30:1-2<99 1992 30 10528 https://doi.org/10.1007/BF00554431 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00554431 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Van Treuren R. Department of Genetics, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands aut NATIONALLICENCE 700 1- Bijlsma R. Department of Genetics, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 30/1-2(1992-02-01), 99-109 0006-2928 30:1-2<99 1992 30 10528 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer