caa a22 4500 469080760 CHVBK 20180323132949.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF01037590 doi (NATIONALLICENCE)springer-10.1007/BF01037590 Isolation of a Drosophila gene encoding glutathione S -transferase [Elektronische Daten] [Clifford Beall, Christine Fyrberg, Sun Song, Eric Fyrberg] We have isolated aDrosophila gene,DmGST-2, that encodes glutathioneS-transferase, a homo- or heterodimeric enzyme thought to be involved in detoxification of xenobiotics, including known carcinogens. The encoded protein has a primary sequence that is more similar to mammalian placental and nematode GSTs than that of a previously describedDrosophila GST gene, herein referred to asDmGST-1. We provide a physical map of the gene and show that it specifies at least two mRNAs, measuring 1.9 and 1.6 kb, which differ only in the lengths of their 3′ untranslated regions. Both of the mRNAs are present during all developmental stages.In situ hybridization of theDmGST-2 gene to larval polytene chromosomes places it within the 53F subdivision of chromosome 2, and Southern blotting to chromosomal DNA indicates that the gene has no close relatives within theDrosophila genome. Our results make possible molecular genetic approaches for further elaborating the function of glutathioneS-transferases in insect development and physiology, in the metabolism of plant toxins, and in conferring insecticide resistance. Plenum Publishing Corporation, 1992 glutathione S -transferase nationallicence Drosophila nationallicence cellular detoxification nationallicence pesticide resistance nationallicence insect metabolism nationallicence Beall Clifford Department of Biology, The Johns Hopkins University, 3400 North Charles Street, 21218, Baltimore, Maryland aut Fyrberg Christine Department of Biology, The Johns Hopkins University, 3400 North Charles Street, 21218, Baltimore, Maryland aut Song Sun Department of Biology, The Johns Hopkins University, 3400 North Charles Street, 21218, Baltimore, Maryland aut Fyrberg Eric Department of Biology, The Johns Hopkins University, 3400 North Charles Street, 21218, Baltimore, Maryland aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 30/9-10(1992-10-01), 515-527 0006-2928 30:9-10<515 1992 30 10528 https://doi.org/10.1007/BF01037590 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01037590 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Beall Clifford Department of Biology, The Johns Hopkins University, 3400 North Charles Street, 21218, Baltimore, Maryland aut NATIONALLICENCE 700 1- Fyrberg Christine Department of Biology, The Johns Hopkins University, 3400 North Charles Street, 21218, Baltimore, Maryland aut NATIONALLICENCE 700 1- Song Sun Department of Biology, The Johns Hopkins University, 3400 North Charles Street, 21218, Baltimore, Maryland aut NATIONALLICENCE 700 1- Fyrberg Eric Department of Biology, The Johns Hopkins University, 3400 North Charles Street, 21218, Baltimore, Maryland aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 30/9-10(1992-10-01), 515-527 0006-2928 30:9-10<515 1992 30 10528 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer