caa a22 4500 469081651 CHVBK 20180323132952.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00338067 doi (NATIONALLICENCE)springer-10.1007/BF00338067 Testosterone and FSH have independent, synergistic and stage-dependent effects upon spermatogenesis in the rat testis [Elektronische Daten] [J. Kerr, S. Maddocks, R. Sharpe] Summary: Adult rats were hypophysectomized and treated with ethane dimethanesulphonate (EDS) selectively to eliminate the Leydig cells in the testis. By removing the source of endogenous gonadotrophins and androgens, the subsequent effects on the seminiferous epithelium were studied after 20 days of treatment with vehicle, or FSH (2x50 μg/day) or a low dose of testosterone (0.6 mg testosterone esters every 3rd day) alone or in combination. Compared to vehicle-treated hypophysectomized rats with Leydig cells, testis weight in saline-treated hypophysectomized rats treated with EDS declined by 50%, spermatogenesis was disrupted severely and only 18% of the tubules contained spermatids, these being confined to stages I-VI of the spermatogenic cycle. Treatment with either FSH or testosterone esters alone significantly (P<0.01) increased testis weight compared to vehicle-treated hypophysectomized rats treated with EDS and 40% of tubules contained spermatids either at stages I-VI after FSH, or at all stages I-XIV after testosterone treatment. Treatment with FSH and testosterone esters together maintained testis weights approximately 20% above vehicle-treated hypophysectomized controls; over 70% of the seminiferous tubules contained spermatids and there was a marked stimulation of spermatogenesis at all stages of the spermatogenic cycle. The results suggest, that in the absence of the pituitary gland and the Leydig cells, FSH alone partially supports spermatogenesis up to the development of round spermatids whereas testosterone is capable of maintaining spermatid development at all 14 stages of the cycle. When FSH and testosterone were administered in combination, the effects upon spermatogenesis were far greater than the response expected if their individual effects were simply additive. It is therefore concluded that FSH may play a role in normal spermatogenesis and that this role is essentially that of augmenting the response of the testis to testosterone. The biochemical mechanisms via which this might occur are discussed and hypophysectomized rats treated with EDS used in the present studies should provide a useful approach for their identification. Springer-Verlag, 1992 Testis nationallicence Spermatogenesis nationallicence FSH nationallicence Testosterone nationallicence Rat (Sprague-Dawley) nationallicence Kerr J. Department of Anatomy, Monash University, Wellington Road, 3168, Clayton, Melbourne, Victoria, Australia aut Maddocks S. Department of Animal Sciences, Waite Agricultural Research Institute, 5064, Glen Osmond, South Australia, Australia aut Sharpe R. MRC Reproductive Biology Unit, Centre for Reproductive Biology, 37 Chalmers Street, EH3 9EW, Edinburgh, UK aut Cell and Tissue Research Springer-Verlag 268/1(1992-04-01), 179-189 0302-766X 268:1<179 1992 268 441 https://doi.org/10.1007/BF00338067 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00338067 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kerr J. Department of Anatomy, Monash University, Wellington Road, 3168, Clayton, Melbourne, Victoria, Australia aut NATIONALLICENCE 700 1- Maddocks S. Department of Animal Sciences, Waite Agricultural Research Institute, 5064, Glen Osmond, South Australia, Australia aut NATIONALLICENCE 700 1- Sharpe R. MRC Reproductive Biology Unit, Centre for Reproductive Biology, 37 Chalmers Street, EH3 9EW, Edinburgh, UK aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/1(1992-04-01), 179-189 0302-766X 268:1<179 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer