caa a22 4500 469081694 CHVBK 20180323132952.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00338065 doi (NATIONALLICENCE)springer-10.1007/BF00338065 Cytoskeletal changes accompanying ACTH-induced steroidogenesis in cultured embryonic adrenal gland cells from the Pekin duck [Elektronische Daten] [James Cronshaw, B. Reese, M. Collie, W. Holmes] Summary: Cells derived from the adrenal glands of duck embryos immediately prior to hatching were grown in culture and used to study the morphological and cytoskeletal changes and steroidogenic responses induced by 1-24 ACTH. Changes in the cytoskeletal components were observed by rhodamine-phalloidin staining for actin and by staining the tubulin immunoreactive components with FITC. The cultures were comprised of a small population of chromaffin cells and a larger population of steroidogenic cells. The chromaffin cells were distinguished by their tyrosine hydroxylase immunoreactivity. The steroidogenic cells were characterized by the presence of sudanophilic lipid droplets, numerous mitochondria, abundant smooth endoplasmic reticulum, microtubules distributed as a fairly even network throughout the cytoplasm, and microfilaments that formed an extensive and elaborate system of stress fibers with many parallel arrays. The cells readily responded to stimulation with ACTH by releasing corticosterone, aldosterone and deoxycorticosterone. Stimulation with ACTH also induced changes in both the cell morphology and the cytoskeleton. Exposure of the cells to Krebs-Henseleit buffer containing 1-24 ACTH caused them to form numerous fine filopodia, to lose their stress fibers, and to form a thick ring of actin at the periphery of the cell. In addition, many cells became extremely arborized with many long branched dendritic processes. The morphological changes appeared to be related to a redistribution of the actin components, and may be explained only in part by the rounding up or retraction of the cytoplasm. The results strongly suggest an involvement of the actin components of the cytoskeleton in the steroidogenic response to corticotropic stimulation. Springer-Verlag, 1992 ACTH nationallicence Actin nationallicence Adrenal gland nationallicence Cell culture nationallicence Corticosterone nationallicence Cytoskeleton nationallicence Steroidogenesis nationallicence Tubulin nationallicence Development, ontogenetic nationallicence Domestic mallard nationallicence Cronshaw James Department of Biological Sciences and the Marine Science Institute, University of California, 93106, Santa Barbara, CA, USA aut Reese B. Department of Biological Sciences and the Marine Science Institute, University of California, 93106, Santa Barbara, CA, USA aut Collie M. Department of Biological Sciences and the Marine Science Institute, University of California, 93106, Santa Barbara, CA, USA aut Holmes W. Department of Biological Sciences and the Marine Science Institute, University of California, 93106, Santa Barbara, CA, USA aut Cell and Tissue Research Springer-Verlag 268/1(1992-04-01), 157-165 0302-766X 268:1<157 1992 268 441 https://doi.org/10.1007/BF00338065 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00338065 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cronshaw James Department of Biological Sciences and the Marine Science Institute, University of California, 93106, Santa Barbara, CA, USA aut NATIONALLICENCE 700 1- Reese B. Department of Biological Sciences and the Marine Science Institute, University of California, 93106, Santa Barbara, CA, USA aut NATIONALLICENCE 700 1- Collie M. Department of Biological Sciences and the Marine Science Institute, University of California, 93106, Santa Barbara, CA, USA aut NATIONALLICENCE 700 1- Holmes W. Department of Biological Sciences and the Marine Science Institute, University of California, 93106, Santa Barbara, CA, USA aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/1(1992-04-01), 157-165 0302-766X 268:1<157 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer