caa a22 4500 469081716 CHVBK 20180323132952.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00338063 doi (NATIONALLICENCE)springer-10.1007/BF00338063 Renin processing studied by immunogold localization of prorenin and renin in granular juxtaglomerular cells in mice treated with enalapril [Elektronische Daten] [Jennifer Berka, Daine Alcorn, Graeme Ryan, Sandford Skinner] Summary: Immunogold techniques were used to investigate renin processing within granular juxtaglomerular cells following short-term (6 h and 1 day) and long-term (4 weeks) enalapril treatment in female BALB/c mice. In control animals, renin protein labelling was localized to all types of granules (proto-, polymorphous, intermediate and mature) and to transport vesicles, whilst prorenin labelling was found in all these sites except mature granules, confirming that active renin is localized to mature granules only. Following short-term enalapril treatment, the exocytosis of renin protein from mature granules was increased. Long-term enalapril treatment resulted in increased numbers of transport vesicles and all types of granules, consistent with increased synthesis and storage of renin. More large intermediate granules contained discrete regions labelled for prorenin. Renin protein was exocytosed from individual and multiple granules, whilst prorenin was exocytosed from protoand intermediate granules. It is concluded that under normal conditions prorenin is secreted constitutively by bulk flow from transport vesicles. On the other hand, active renin is secreted regulatively from mature granules. In conditions of intense stimulation (angiotensin-converting enzyme inhibition treatment), increased synthesis of prorenin leads to enhanced secretion of prorenin by both constitutive and regulative pathways. Under these conditions, the conversion of prorenin to active renin is increased, with increased secretion of active renin occurring in a regulative manner. Furthermore, the localization of prorenin to one discrete region of large intermediate granules leads us to conclude, that cleavage of the prosegment of renin occurs with the transition of intermediate to mature granules. Springer-Verlag, 1992 Prorenin nationallicence Renin nationallicence Granular juxtaglomerular cell nationallicence Immunogold technique nationallicence Exocytosis nationallicence Mouse (BALB/c) nationallicence Berka Jennifer Department of Anatomy, University of Melbourne, 3052, Parkville, Victoria, Australia aut Alcorn Daine Department of Anatomy, University of Melbourne, 3052, Parkville, Victoria, Australia aut Ryan Graeme Department of Anatomy, University of Melbourne, 3052, Parkville, Victoria, Australia aut Skinner Sandford Department of Physiology, University of Melbourne, 3052, Parkville, Victoria, Australia aut Cell and Tissue Research Springer-Verlag 268/1(1992-04-01), 141-148 0302-766X 268:1<141 1992 268 441 https://doi.org/10.1007/BF00338063 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00338063 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Berka Jennifer Department of Anatomy, University of Melbourne, 3052, Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Alcorn Daine Department of Anatomy, University of Melbourne, 3052, Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Ryan Graeme Department of Anatomy, University of Melbourne, 3052, Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Skinner Sandford Department of Physiology, University of Melbourne, 3052, Parkville, Victoria, Australia aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/1(1992-04-01), 141-148 0302-766X 268:1<141 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer