caa a22 4500 469081732 CHVBK 20180323132952.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00338069 doi (NATIONALLICENCE)springer-10.1007/BF00338069 Alterations in cytokeratin expression precede histological changes in epithelia of vitamin A-deficient rats [Elektronische Daten] [M. Gijbels, F. van der Ham, A. van Bennekum, H. Hendriks, P. Roholl] Summary: Normal epithelial cell differentiation is charactezied by the production of distinct cytokeratin proteins. It is well known that epithelia of several organs show squamous metaplasia in a vitamin A-deficient status. It is not yet known whether these histological changes are concomitant with a change in cytokeratin expression. Therefore, 3-week-old female rats (BN/BiRij) were fed a vitamin A-deficient diet for 8 weeks. The cytokcratin expression in epithelia of various organs was monitored immunohistochemically during the induction of vitamin A deficiency. Therefore, monoclonal antibodies specific for human cytokeratin 4, 5, 5+8, 7, 10, 14, 18 and 19 were used. In a normal vitamin A status, the distributional pattern for the different cytokeratins in rats was similar to that reported for human tissue. No change in cytokeratin expression was seen in trachea, skin, liver and colon at any time point studied. Squamous metaplasia in urinary bladder and salivary glands was observed after six weeks on the vitamin A-deficient diet. This was concomitant with a substitution of cytokeratins 4, 5+8, 7, 18 and 19 by cytokeratin 10. The latter cytokeratin is specific for keratinzed squamous epithelium. A change in cytokeratin expression was observed in bladder, ureter, kidney, salivary glands, uterus and conjunctiva before histological alterations appeared. In conclusion, the changes in cytokeratin expression observed under vitamin A deficiency in epithelia in vivo are in agreement with those described in other studies for epithelial cells in vitro. The changes in cytokeratin expression and the subsequent differentiation into squamous cells occurs in basal cells of the bladder but not in transitional cells. Furthermore, histological alterations are preceded by changes in cytokeratin expression indicating that vitamin A status controls cytokeratin expression in vivo. Springer-Verlag, 1992 Vitamin A deficiency nationallicence Cytokeratins nationallicence Epithelial cells nationallicence Immunohistochemistry nationallicence Rat (BN/BiRij) nationallicence Gijbels M. TNO Institute of Ageing and Vascular Research IVVO, P.O. Box 430, NL-2300 AK, Leiden, The Netherlands aut van der Ham F. TNO Institute of Ageing and Vascular Research IVVO, P.O. Box 430, NL-2300 AK, Leiden, The Netherlands aut van Bennekum A. TNO Institute of Ageing and Vascular Research IVVO, P.O. Box 430, NL-2300 AK, Leiden, The Netherlands aut Hendriks H. TNO Institute of Ageing and Vascular Research IVVO, P.O. Box 430, NL-2300 AK, Leiden, The Netherlands aut Roholl P. TNO Institute of Ageing and Vascular Research IVVO, P.O. Box 430, NL-2300 AK, Leiden, The Netherlands aut Cell and Tissue Research Springer-Verlag 268/1(1992-04-01), 197-203 0302-766X 268:1<197 1992 268 441 https://doi.org/10.1007/BF00338069 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00338069 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gijbels M. TNO Institute of Ageing and Vascular Research IVVO, P.O. Box 430, NL-2300 AK, Leiden, The Netherlands aut NATIONALLICENCE 700 1- van der Ham F. TNO Institute of Ageing and Vascular Research IVVO, P.O. Box 430, NL-2300 AK, Leiden, The Netherlands aut NATIONALLICENCE 700 1- van Bennekum A. TNO Institute of Ageing and Vascular Research IVVO, P.O. Box 430, NL-2300 AK, Leiden, The Netherlands aut NATIONALLICENCE 700 1- Hendriks H. TNO Institute of Ageing and Vascular Research IVVO, P.O. Box 430, NL-2300 AK, Leiden, The Netherlands aut NATIONALLICENCE 700 1- Roholl P. TNO Institute of Ageing and Vascular Research IVVO, P.O. Box 430, NL-2300 AK, Leiden, The Netherlands aut NATIONALLICENCE 773 0- Cell and Tissue Research Springer-Verlag 268/1(1992-04-01), 197-203 0302-766X 268:1<197 1992 268 441 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer